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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin
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Specifications:
| Application | Protein Assays & Analysis, Colorimetric Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | Formula | C₁₄H₁₈N₃NaO₈S₂ |
Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin is a compact, water-soluble biotinylation reagent designed for fast, efficient labeling of antibodies, proteins, and other amine-containing macromolecules. With a short 13.5 Å spacer and a sulfonated NHS ester, this reagent is particularly well-suited for cell-impermeable applications such as labeling of extracellular proteins and surface-bound targets in aqueous environments.
Key Features and Benefits
Efficient Amine Reactivity
- Contains a sulfo-NHS ester that forms stable, irreversible amide bonds with primary amines on lysine side chains or protein N-termini.
Short Spacer Arm (13.5 Å)
- Consists solely of biotin's native valeric acid group for minimal structural modification.
- Ideal when minimal distance between biotin and the target is desired to preserve functionality.
Water Solubility & Cell Impermeability
- Highly water-soluble; does not require organic solvents.
- Membrane-impermeable due to its negatively charged sulfo group, making it suitable for surface protein labeling only.
Stable & Versatile
- Forms permanent, non-cleavable amide bonds.
- Suitable for a wide variety of biomolecules, including proteins, peptides, and enzymes.
Applications
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Biotinylation of antibodies for:
- ELISA
- Western blotting
- Immunoprecipitation
- Flow cytometry
- Cell surface labeling of live or fixed cells
- Affinity purification or capture of biotinylated proteins using streptavidin/avidin resins
- Bioconjugation in aqueous buffer systems for highly sensitive detection assays
Technical Specifications
| Property | Details |
|---|---|
| Catalog Numbers | 21217 (50 mg), A39256 (10 × 1 mg) |
| Reagent Type | Sulfo-NHS-Biotin |
| Spacer Arm Length | 13.5 Å (short) |
| Reactive Moiety | Sulfo-NHS ester |
| Target Functional Group | Primary amines (-NH₂) |
| Solubility | Water, DMSO, DMF |
| Cell Permeability | No (cell-impermeant) |
| Label Type | Biotin |
| Formulation | Solid |
| Storage Conditions | Store desiccated at –20°C |
| Shipping Condition | Ambient temperature |
| Unit Size | 50 mg or 10 × 1 mg |
EZ-Link™ Sulfo-NHS-Biotin is a robust, short-chain biotinylation reagent ideal for targeted, aqueous-based protein labeling. Its small spacer arm enables close-proximity interactions, while the sulfo group ensures high solubility and surface-selectivity in cellular and protein assays. Whether used in antibody conjugation, surface biotinylation, or protein detection workflows, this reagent provides high-performance results in biotin-streptavidin affinity applications.
- Pack Size: 50 mg 10 x 1 mg