Thermo Scientific™ Bromophenol Blue, ACS Grade

Thermo Scientific™ Bromophenol Blue, ACS is a high-purity analytical-grade dye widely used as a tracking dye, biological stain, and pH indicator in molecular biology, biochemistry, and analytical chemistry applications. With ACS-grade purity and consistent performance, it provides reliable visualization of electrophoresis progress and accurate pH indication within the acidic transition range of pH 3.0 to 4.6.

Bromophenol Blue is particularly valued in DNA, RNA, and protein electrophoresis workflows, where it functions as a migration marker, allowing researchers to monitor sample movement during agarose or polyacrylamide gel electrophoresis. Its distinct color transition from yellow (acidic) to blue (basic) makes it equally useful as an acid-base indicator in laboratory titrations and biochemical assays.

Manufactured to strict ACS specifications, this reagent ensures excellent solution clarity, high reproducibility, and compatibility with sensitive analytical and molecular biology techniques.

Key Features and Benefits

ACS Analytical Grade Purity

• Certified to American Chemical Society (ACS) standards
• High consistency and reliability for analytical and research applications

Ideal Electrophoresis Tracking Dye

• Commonly used in:
  • Agarose gel electrophoresis
  • Polyacrylamide gel electrophoresis (PAGE)
  • SDS-PAGE protein analysis
• Allows real-time visualization of sample migration

Effective pH Indicator

• Clear visual transition:
  • Yellow at pH 3.0
  • Blue at pH 4.6
• Suitable for acidic pH range monitoring and titration experiments

Biological Staining Applications

• Used for staining proteins and nucleic acids
• Supports visualization in electrophoresis and analytical workflows

High Chemical Stability and Purity

• Excellent solution clarity
• Reliable performance in sensitive assays

Applications

• DNA electrophoresis tracking dye
• RNA electrophoresis workflows
• Protein electrophoresis (SDS-PAGE, PAGE)
• Molecular biology and genomics workflows
• pH indicator in analytical chemistry
• Biological staining of proteins and nucleic acids
• Buffer preparation and laboratory titration
• Biochemical and analytical laboratory research

Available Models and Ordering Information

Chemical and Physical Properties

Typical Workflow Integration

Electrophoresis Sample Preparation → Add Loading Buffer with Bromophenol Blue → Gel Electrophoresis Run → Track Migration Progress → Protein/DNA/RNA Visualization

Storage and Handling

• Store at room temperature in a dry environment
• Protect from moisture and direct light
• Ensure container is tightly sealed after use

Thermo Scientific™ Bromophenol Blue, ACS Grade is a highly reliable and essential reagent for electrophoresis tracking, biological staining, and pH indication. Its high purity, consistent performance, and clear color transition make it an indispensable component in molecular biology, electrophoresis, and analytical chemistry laboratories requiring accurate visualization and pH monitoring.