0
SuperSignal™ West Pico PLUS Chemiluminescent Substrate
Catalog No :
CAS Number :
Brand :
In Stock
an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an advanced enhanced chemiluminescent (ECL) detection reagent designed for picogram to high femtogram-level protein detection in western blot applications. This substrate delivers exceptional signal intensity and extended duration, enabling multiple exposures and high-quality imaging for publication-ready results.
Its broad compatibility with nitrocellulose and PVDF membranes, various blocking reagents, and wide antibody dilution ranges makes it a reliable choice for routine and high-sensitivity immunoblotting.
Key Features & Benefits
- High Sensitivity – Detect proteins at picogram to high femtogram levels for maximum clarity.
- Extended Signal Duration – Stable chemiluminescent signal for 6 to 24 hours, allowing multiple exposures.
- Excellent Stability – 8-hour working solution stability; 1-year kit stability at room temperature.
- Broad Compatibility – Works with both nitrocellulose and PVDF membranes and multiple blocking systems.
- Flexible Antibody Dilutions – Primary: 1:1000–1:5000 (0.2–1.0 μg/mL); Secondary: 1:20,000–100,000 (10–50 ng/mL).
- Easy Integration – Compatible with existing HRP-based western blotting workflows.
Applications
- Western Blotting – High-sensitivity detection of HRP-conjugated antibodies.
- Protein Expression Analysis – Ideal for detecting low-abundance proteins.
- Multiple Exposure Imaging – Capture high-resolution images over extended periods.
Technical Specifications
| Property | Details |
|---|---|
| Detection Method | Chemiluminescence |
| Sensitivity | Picogram to high femtogram-level |
| Signal Duration | 6–24 hours |
| Stability | Working solution: 8 hours; Kit: 1 year at room temperature |
| Conjugate | Horseradish Peroxidase (HRP) |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Form | Liquid |
| Storage | Room temperature |
| Recommended Primary Antibody Dilution | 1:1000–1:5000 (0.2–1.0 μg/mL) |
| Recommended Secondary Antibody Dilution | 1:20,000–100,000 (10–50 ng/mL) |
| Kit Contents | SuperSignal West Pico PLUS Luminol/Enhancer, SuperSignal West Pico PLUS Stable Peroxide |
| For Use With | Research applications only |
Ordering Information
| Catalog Number | Quantity |
|---|---|
| 34579 | 20 mL |
| 34577 | 200 mL |
| 34580 | 500 mL |
| 34580X4 | 4 × 500 mL |
| 34578 | 1 L |
| 34578X4 | 4 × 1 L |
Storage: Store at room temperature.
Regulatory Status: For Research Use Only – Not for use in diagnostic procedures.
The Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate offers a dependable, high-sensitivity solution for western blot detection, enabling researchers to achieve precise, reproducible, and publication-quality results. Its combination of long-lasting signal, broad compatibility, and excellent sensitivity makes it an ideal choice for both routine and advanced protein detection workflows. Whether working with abundant or low-expression targets, this substrate ensures accurate visualization with minimal background and maximum clarity.
- Pack Size: 1L 500 mL 200 mL 20 mL 4 x 500 mL 4 x 1 L