SARS-CoV-2 Spike RBD Protein, His Tag (JN.1/Omicron) (MALS verified), >95%, 100 µg

It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source

SARS-CoV-2 Spike RBD Protein, His Tag (JN.1/Omicron) (SPD-C5249) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (I332V,G339H,K356T,S371F,S373P,S375F,T376A,R403K,D405N,R408S,K417N,N440K,V445H,G446S,N450D,L452W,L455S,N460K,S477N,T478K,N481K,V483del,E484K,F486P,Q498R,N501Y,Y505H)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: JN.1/Omicron).

Predicted N-terminus: Arg 319

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• EndotoxinLess than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:

SDS-PAGE

SARS-CoV-2 Spike RBD Protein, His Tag (JN.1/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS

The purity of SARS-CoV-2 Spike RBD Protein, His Tag (JN.1/Omicron) (Cat. No. SPD-C5249) is more than 90% and the molecular weight of this protein is around 32-45 kDa verified by SEC-MALS.

Bioactivity-ELISA

Immobilized SARS-CoV-2 Spike RBD Protein, His Tag (JN.1/Omicron) (Cat. No. SPD-C5249) at 5 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.1-2 ng/mL (QC tested).

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD Protein, His Tag (JN.1/Omicron) (Cat. No. SPD-C5249) with a linear range of 0.008-0.25 μg/mL (Routinely tested).