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Invitrogen™ Novex™ AP Mouse Chemiluminescent Detection Kit
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Sufficient for 10 Mini Membranes
Specifications:
| Application | Western Blot | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
High-sensitivity alkaline phosphatase-based chemiluminescent detection for mouse primary antibodies
The Invitrogen™ Novex™ AP Mouse Chemiluminescent Detection Kit delivers ultra-sensitive, long-lasting protein detection in western blotting applications using alkaline phosphatase (AP) conjugation chemistry.
Optimized for use with nitrocellulose or PVDF membranes, the kit includes a secondary AP-conjugated anti-mouse antibody, ready-to-use CDP-Star™ chemiluminescent substrate, and an enhancer. It is designed for compatibility with most western blot protocols, with optimal performance on the iBind™ Western System.
With a straightforward workflow, the kit enables detection in less than 5 minutes, produces clean backgrounds, and provides signals that last up to 5 days, making it ideal for applications requiring re-imaging or prolonged exposure.
Key Features & Benefits
- Ultra-Sensitive Detection – Achieves high signal intensity for low-abundance proteins.
- Rapid Results – Visualize results in under 5 minutes.
- Long-Lasting Signals – Stable chemiluminescence for up to 5 days.
- High Specificity – AP-conjugated anti-mouse antibody ensures minimal background noise.
- Complete Kit – Includes secondary antibody, substrate, and enhancer for 10 mini-membranes.
- Versatile Membrane Compatibility – Works with both nitrocellulose and PVDF membranes.
- Optimized for iBind™ – Ideal for automated, hands-free western blot workflows.
Applications
- Western blot detection of proteins using mouse primary antibodies
- High-sensitivity protein expression analysis
- Quantitative and qualitative protein detection in research workflows
- Long-exposure or re-imaging western blot applications
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Western blot chemiluminescent detection kit |
| Product Line | Novex™ |
| Format | Kit |
| Reactivity | Mouse |
| Sample Type | Protein |
| Species | Mouse |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Detection Method | Chemiluminescence |
| Substrate Type | AP (Alkaline Phosphatase) Substrate |
| Substrate | CDP-Star™ AP substrate |
| Includes | Secondary AP-conjugated anti-mouse antibody, CDP-Star™ substrate, enhancer |
| Sufficient For | 10 mini-membranes (iBind™ Western System) |
| For Use With | iBind™ Western System, compatible with most western blot protocols |
| Results Time | <5 minutes |
| Signal Duration | Up to 5 days |
| Storage Conditions | 2–4°C |
| Catalog Number | SLF1021 |
| Unit Size | 1 kit |
The Invitrogen™ Novex™ AP Mouse Chemiluminescent Detection Kit provides researchers with a fast, highly sensitive, and long-lasting detection solution for western blotting with mouse primary antibodies. Offering compatibility with both nitrocellulose and PVDF membranes, and optimized for the iBind™ Western System, it ensures sharp, low-background results in minutes. Its robust signal stability and complete reagent package make it an excellent choice for high-quality, reproducible protein detection in molecular biology research.