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Specifications:
| Application | Western Blot | ||
| Storage Temperature | Room Temperature, 2-8°C | ||
| Product Type | Molecular Biology Reagent, Microbiology Reagent | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Complete package for automated western blot processing
The Invitrogen™ iBind™ Western Starter Kit provides everything needed to begin automated western blot processing using the iBind™ Western Device. This kit includes the device, consumables, and reagents required to perform every step of a western blot—from blocking to washes to antibody incubations—via sequential lateral flow (SLF) technology.
The iBind™ Western Device operates without external power, shakers, or trays. Solutions and antibodies are released in a timed sequence from the device wells to an iBind™ Card, which evenly distributes reagents across the membrane via a glass fiber matrix. This ensures uniform antigen–antibody interactions and consistent, reproducible results.
The kit processes one mini blot (8 × 8 cm) per run in approximately 2.5 hours and is compatible with nitrocellulose and PVDF membranes, supporting chromogenic, chemiluminescent, and fluorescent detection methods.
Key Features & Benefits
- All-in-One Package – Includes device, cards, and solution kit for immediate setup and use.
- Fully Automated Workflow – Performs blocking, antibody incubation, and wash steps hands-free.
- Sequential Lateral Flow Technology – Ensures consistent, timed reagent delivery.
- High Reproducibility – Minimizes variability compared to manual methods.
- Power-Free Operation – No external power source or additional equipment required.
- Versatile Detection – Supports fluorescence, chemiluminescence, and chromogenic detection.
- Membrane Compatibility – Works with both nitrocellulose and PVDF membranes.
Applications
- Automated western blot protein detection
- Fluorescent, chemiluminescent, and chromogenic immunodetection workflows
- Hands-free western blot processing in research labs
- Consistent antibody incubation protocols
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Starter Kit |
| Product Line | iBind™ |
| Capacity | 1 mini blot (8 × 8 cm) |
| Detection Methods | Chromogenic, Chemiluminescence, Fluorescence |
| Membrane Compatibility | Nitrocellulose, PVDF |
| Run Time | ~2.5 hours |
| Throughput | 1 mini blot per run |
| Includes | 1 iBind™ Western Device, 10 iBind™ Cards, 1 iBind™ Solution Kit |
| Unit Size | 1 kit |
| Quantity | 1 kit |
| Storage Conditions | Device and cards at room temperature; Solution kit at 2–8°C |
| Catalog Number | SLF1000S |
The Invitrogen™ iBind™ Western Starter Kit is the ideal choice for researchers seeking a complete, ready-to-use solution for automated western blot processing. With all essential components included, it enables immediate adoption of sequential lateral flow technology, reducing hands-on time, improving reproducibility, and supporting multiple detection methods for versatile protein analysis.