Human Aldosterone (ALD) ELISA Kit

The Human Aldosterone (ALD) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of Aldosterone (ALD) levels in human serum, plasma, culture media, or other biological fluids. This kit uses a Sandwich-ELISA method to provide reliable and accurate results for research purposes.

Purpose

• Quantification of Human Aldosterone (ALD):
  • Used in biological and clinical research to study aldosterone levels.
• Applicable Samples:
  • Human serum, plasma, culture media, or other biological fluids.

Principle

This kit employs the Sandwich-ELISA technique to ensure precision and reliability. The workflow is detailed below:

1. Pre-coated Plate:
   • The provided Microelisa strip plate is pre-coated with an antibody specific to human ALD.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing ALD to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific for ALD is added, forming a complex with ALD in the sample.
4. Washing Step:
   • Unbound components are washed away to eliminate background noise.
5. TMB Substrate Reaction:
   • TMB substrate is added, producing a blue color in the presence of HRP.
6. Stop Reaction:
   • A stop solution is added, converting the color from blue to yellow.
7. Optical Density Measurement:
   • The optical density (OD) is measured at 450 nm using a spectrophotometer. The OD is directly proportional to the concentration of ALD in the sample.

Features

1. High Sensitivity and Specificity:
   • Detects low levels of ALD with minimal cross-reactivity.
2. Quantitative Results:
   • Generates a standard curve for precise measurement of ALD concentrations.
3. Wide Sample Compatibility:
   • Suitable for a variety of biological fluids, including serum and plasma.
4. Ready-to-Use Components:
   • Pre-coated plates and reagents simplify assay setup.
5. Reproducibility:
   • Consistent results across multiple experiments due to stringent quality control.

Kit Components

• Pre-coated Microelisa strip plate.
• ALD standards for generating a standard curve.
• HRP-conjugated detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Endocrinological Research:
  • Study aldosterone's role in regulating blood pressure and electrolyte balance.
• Clinical Research:
  • Investigate conditions such as hyperaldosteronism or adrenal gland disorders.
• Pharmacological Studies:
  • Evaluate drug effects on aldosterone production or activity.
• Hormonal Regulation Studies:
  • Explore aldosterone's impact on the renin-angiotensin-aldosterone system (RAAS).

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated ALD antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate ALD concentration using the standard curve.

Storage and Stability

• Store kit at 2–8°C to maintain reagent stability.
• Ensure all reagents are equilibrated to room temperature before use.

Advantages

• Ease of Use:
  • User-friendly protocol with pre-coated plates and ready-to-use reagents.
• Rapid Detection:
  • Quick incubation times streamline the process.
• High Precision:
  • Accurate quantification of ALD levels in biological fluids.

The Human Aldosterone (ALD) ELISA Kit provides a reliable and efficient method for measuring ALD in a wide range of biological samples. Its high sensitivity, specificity, and straightforward protocol make it an essential tool for research in endocrinology, clinical studies, and hormonal regulation.