Human Citrate Synthase (Cs)Elisa Kit

The Human Citrate Synthase (CS) ELISA Kit is a sensitive and reliable assay designed for the quantitative detection of CS levels in human serum, plasma, culture media, and other biological fluids. Using a Sandwich-ELISA technique, this kit is ideal for research in cellular metabolism, mitochondrial function, and related disorders.

Purpose

• Quantification of Citrate Synthase Levels:
  • Measures CS concentrations to evaluate its role as a key enzyme in the citric acid cycle and mitochondrial function.
• Sample Compatibility:
  • Suitable for human serum, plasma, and various biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for specific and accurate measurement of CS. Below is the workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to Citrate Synthase (CS).
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing CS in the sample to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to CS is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with CS.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • CS concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of CS with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of CS concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• CS standards for standard curve generation.
• HRP-conjugated CS detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Mitochondrial Function Research:
  • Study CS as a marker of mitochondrial activity and biogenesis.
• Cellular Metabolism Studies:
  • Investigate the role of CS in the citric acid cycle and energy production.
• Biomarker Detection:
  • Quantify CS levels in metabolic and mitochondrial disorders.
• Drug Development:
  • Evaluate the effects of drugs targeting mitochondrial pathways.
• Disease Research:
  • Monitor CS levels in conditions such as cancer, neurodegenerative diseases, and metabolic disorders.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of CS.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated CS antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate CS concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Citrate Synthase (CS) ELISA Kit is a vital tool for measuring CS levels in biological samples. Its high sensitivity, specificity, and user-friendly design make it indispensable for research in cellular metabolism, mitochondrial function, and related diseases.