Human Chorionic Gonadotrophin, HCG ELISA Kit

Human Chorionic Gonadotropin (HCG) ELISA Kit

The Human Chorionic Gonadotropin (HCG) ELISA Kit is a sensitive and specific assay designed for the quantitative measurement of HCG levels in human serum, plasma, culture media, and other biological fluids. Employing the Sandwich-ELISA technique, this kit is an invaluable tool for research in pregnancy, reproductive health, and related conditions.

Purpose

• Quantification of HCG Levels:
  • Measures HCG concentrations to assess its role in pregnancy, fertility, and hormone-related studies.
• Sample Compatibility:
  • Suitable for human serum, plasma, and various biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique for the accurate and specific measurement of HCG. Below is the workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to HCG.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing HCG in the sample to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to HCG is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with HCG.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • HCG concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of HCG with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of HCG concentrations using a standard curve.
4. Ease of Use:
   • Pre-coated plates and ready-to-use reagents simplify the workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• HCG standards for standard curve generation.
• HRP-conjugated HCG detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Pregnancy Research:
  • Investigate HCG as a marker for early pregnancy detection and monitoring.
• Reproductive Health Studies:
  • Study HCG’s role in fertility and assisted reproductive technologies.
• Hormonal Regulation Research:
  • Explore HCG in endocrine function and its influence on hormone levels.
• Cancer Research:
  • Assess HCG as a biomarker for certain cancers, such as testicular and ovarian cancers.
• Drug Development:
  • Evaluate therapeutic interventions targeting HCG or related pathways.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of HCG.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated HCG antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate HCG concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Chorionic Gonadotropin (HCG) ELISA Kit provides a sensitive, accurate, and user-friendly solution for quantifying HCG in biological samples. Its specificity and versatility make it an essential tool for pregnancy research, reproductive health studies, and related clinical and scientific investigations.