Bovine Interferon α(IFN-α)elisa Kit

Bovine Interferon α (IFN-α) ELISA Kit

The Bovine Interferon α (IFN-α) ELISA Kit is a highly sensitive and specific assay designed to quantify IFN-α levels in various biological fluids, including serum, plasma, and culture media. This kit employs a sandwich ELISA method for reliable detection and measurement of bovine IFN-α.

Purpose

• Quantification of Bovine IFN-α:
  • For use in biological research, including immunology and veterinary diagnostics.
• Applicable Samples:
  • Serum, plasma, culture supernatants, and other biological fluids.

Principle

The kit utilizes a Sandwich-ELISA technique to ensure high specificity and sensitivity. Below is a detailed description of the process:

1. Plate Preparation:
   • The Microelisa strip plate is pre-coated with an antibody specific to bovine IFN-α.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing IFN-α to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific for IFN-α is added to the wells, forming a complex with IFN-α.
4. Washing Step:
   • Unbound components are washed away to reduce background noise.
5. TMB Substrate Reaction:
   • TMB substrate is added to the wells. In the presence of HRP, a blue color develops, proportional to the IFN-α concentration.
6. Stop Reaction:
   • A stop solution is added, changing the color to yellow.
7. Optical Density Measurement:
   • The optical density (OD) is measured at 450 nm using a spectrophotometer. The OD value correlates directly with the IFN-α concentration in the sample.

Features

1. High Sensitivity:
   • Detects low levels of bovine IFN-α with precision.
2. Specificity:
   • Pre-coated antibodies ensure minimal cross-reactivity.
3. Wide Applicability:
   • Suitable for a range of sample types, including serum, plasma, and culture media.
4. Quantitative Results:
   • Generates a standard curve for accurate determination of IFN-α concentrations.
5. Ready-to-Use Components:
   • Includes all necessary reagents and pre-coated plates for ease of use.

Kit Components

• Pre-coated Microelisa strip plate.
• IFN-α standards for curve generation.
• HRP-conjugated detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Immunological Research:
  • Study of IFN-α-mediated immune responses in bovines.
• Veterinary Diagnostics:
  • Detection and quantification of IFN-α in clinical samples.
• Infection Monitoring:
  • Assessment of antiviral or immune-modulating responses in research or disease states.
• Cytokine Profiling:
  • Analyze cytokine expression in experimental or field studies.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated IFN-α antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate IFN-α concentration using the standard curve.

Storage and Stability

• Store kit at 2–8°C for long-term stability.
• Ensure all reagents are equilibrated to room temperature before use.

Advantages

• Ease of Use:
  • Simple workflow with pre-coated plates and ready-to-use reagents.
• Reproducibility:
  • High-quality reagents ensure consistent results.
• Rapid Detection:
  • Quick incubation times streamline the process.

The Bovine Interferon α (IFN-α) ELISA Kit provides a reliable and accurate method for measuring IFN-α in various sample types. Its high sensitivity, specificity, and straightforward protocol make it an essential tool for research in immunology, veterinary medicine, and cytokine profiling.