Sigma-Aldrich Vancomycin hydrochloride from Streptomyces orientalis, BioReagent, suitable for plant cell culture

High-Purity Glycopeptide Antibiotic for Microbiology, Cell Culture, and Antimicrobial Research

Sigma-Aldrich Vancomycin Hydrochloride (BioReagent, Cell Culture Grade) is a high-purity glycopeptide antibiotic widely used in microbiology, pharmaceutical research, and plant cell culture. Originally derived from Streptomyces orientalis, Vancomycin effectively inhibits bacterial cell wall synthesis by preventing D-alanyl-D-alanine incorporation into peptidoglycan chains, making it a potent treatment against Gram-positive bacteria.

With a potency of ≥900 μg/mg (as vancomycin base) and validated for cell culture applications, this antibiotic is widely used in bacterial contamination prevention, antimicrobial susceptibility testing, and research on drug-resistant bacterial strains such as MRSA.

Key Features & Benefits

✅ High-Purity BioReagent Grade – Ensures consistent performance in microbiology & plant cell culture applications

✅ Broad-Spectrum Glycopeptide Antibiotic – Effective against Gram-positive bacteria, including MRSA

✅ Cell Culture-Suitable – Used to prevent bacterial contamination in plant & mammalian cell cultures

✅ Mode of Action: Inhibits Peptidoglycan Synthesis – Prevents D-alanyl-D-alanine incorporation in bacterial cell walls

✅ Highly Soluble in Water (50 mg/mL) – Allows easy preparation of antibiotic stock solutions

✅ Validated for Research & Drug Development – Supports antimicrobial resistance studies & pharmaceutical testing

✅ Stable Under Proper Storage Conditions (2–8°C) – Ensures long-term activity retention

Technical Specifications

Mechanism of Action & Resistance

Mode of Action:

• Vancomycin inhibits bacterial cell wall synthesis by binding to D-alanyl-D-alanine terminal residues in peptidoglycan precursors, preventing cross-linking and cell wall formation.
• This disruption weakens the bacterial cell wall, leading to osmotic instability and bacterial lysis.

Mode of Resistance:

• Vancomycin-resistant bacteria (e.g., VRE - Vancomycin-resistant Enterococcus) alter the D-alanyl-D-alanine target site, reducing drug affinity.
• Some bacteria produce thicker peptidoglycan layers, limiting Vancomycin access to its binding site.

Antimicrobial Spectrum:

✔ Gram-Positive Bacteria (Staphylococcus aureus, Streptococcus pneumoniae, Clostridium difficile)

✔ Methicillin-Resistant Staphylococcus aureus (MRSA)

✔ Enterococcus spp. & Other Drug-Resistant Bacteria

Applications

🔬 Microbiology & Bacterial Culture Research – Used for selective inhibition of Gram-positive bacteria

🦠 Antimicrobial Susceptibility Testing (AST) – Evaluates bacterial resistance to Vancomycin

⚗️ Pharmaceutical Drug Development – Supports research on glycopeptide antibiotics & bacterial resistance

🧪 Cell Culture Contamination Prevention – Prevents bacterial contamination in plant & mammalian cell cultures

🏭 Food & Environmental Safety Testing – Used in detection of antibiotic-resistant bacteria in food products

Preparation & Storage Guidelines

1️⃣ Preparation of Stock Solution

• Dissolve 50 mg/mL in sterile water or PBS.
• Filter sterilization recommended (do not autoclave).
• Store at 2–8°C for short-term use or -20°C for extended stability.

2️⃣ Recommended Working Concentrations

• Bacterial Inhibition in Microbiology Studies: 10–100 µg/mL
• Antimicrobial Susceptibility Testing (AST): Variable (refer to CLSI/EUCAST guidelines)

3️⃣ Storage Conditions

• Keep tightly sealed in a dry, ventilated environment.
• Protect from moisture & high temperatures to prevent degradation.
• Avoid repeated freeze-thaw cycles for optimal stability.

Ordering Information

Regulatory & Safety Information

⚠️ For Research Use Only (RUO). Not intended for clinical or therapeutic applications.

⚠️ Hygroscopic & Light-Sensitive – Store in a dry, well-ventilated area.

⚠️ Use Protective Equipment (PPE) – Avoid direct skin contact & inhalation.

Why Choose Sigma-Aldrich Vancomycin Hydrochloride (BioReagent, Cell Culture Grade)?

🔹 High-Purity Antibiotic (≥900 μg/mg) for Reliable Research

🔹 Effective Against Gram-Positive Bacteria, Including MRSA

🔹 Validated for Cell Culture, Pharmaceutical Research, and Antimicrobial Studies

🔹 Stable Under Proper Storage Conditions (2–8°C)

🔹 Available in Multiple Pack Sizes for Research Flexibility

For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀