Sigma-Aldrich Rifampicin ≥95% (HPLC), powder

Broad-Spectrum Antibiotic for Tuberculosis, Mycobacterial Infections & Research Applications

Sigma-Aldrich Rifampicin (≥95% HPLC, Powder) is a high-purity macrolide antibiotic widely used in microbiology, pharmaceutical research, and tuberculosis treatment studies. It is a potent inhibitor of RNA polymerase, effectively targeting Gram-positive and Gram-negative bacteria, mycobacteria, and certain viruses.

This reddish-brown crystalline powder is highly effective in tuberculosis (TB) research, bacterial resistance studies, and RNA synthesis inhibition assays. With its broad-spectrum activity and validated purity (≥95%), Rifampicin is an essential reagent in microbial pathogenesis, pharmaceutical formulations, and molecular biology research.

Key Features & Benefits

✅ High Purity (≥95% by HPLC) – Ensures consistent and reproducible results in research applications

✅ Broad-Spectrum Antibiotic Activity – Effective against Gram-positive, Gram-negative bacteria, and mycobacteria

✅ Validated for TB Research & Microbial Studies – Used in tuberculosis treatment studies & antimicrobial resistance testing

✅ RNA Polymerase Inhibitor – Blocks transcription initiation by binding to the β-subunit of RNA polymerase

✅ Macrolide Antibiotic with Selective Action – Inhibits DNA and protein assembly in bacterial cells

✅ Highly Soluble in Organic Solvents – Enhances formulation flexibility for different applications

✅ Stable Under Controlled Conditions – Requires storage at -20°C to maintain activity

Technical Specifications

Mechanism of Action & Resistance

Mode of Action:

• Rifampicin binds to the β-subunit of bacterial RNA polymerase, preventing the initiation of RNA synthesis.
• This results in transcriptional inhibition and cell death, making it an effective treatment for tuberculosis and other bacterial infections.

Mode of Resistance:

• Resistance arises due to mutations in the rpoB gene, altering the RNA polymerase binding site.
• Some bacteria produce Rifampicin-modifying enzymes that reduce antibiotic effectiveness.

Antimicrobial Spectrum:

✔ Gram-Positive Bacteria (Staphylococcus spp., Streptococcus spp.)

✔ Gram-Negative Bacteria (Neisseria meningitidis, Haemophilus influenzae)

✔ Mycobacteria (Mycobacterium tuberculosis, Mycobacterium leprae)

✔ Certain Viruses – Inhibits DNA and protein assembly in some viral species

Applications

🔬 Tuberculosis (TB) Research & Mycobacterial Studies – Key antibiotic for Mycobacterium tuberculosis resistance studies

🦠 Microbial Pathogenesis & Bacterial RNA Synthesis Studies – Evaluates RNA polymerase inhibition in bacterial transcription

⚗️ Pharmaceutical Formulation & Antibiotic Development – Used in drug formulation & antimicrobial resistance research

🧪 Microbial Resistance Mechanisms & Gene Expression – Studies mutations in the rpoB gene for Rifampicin resistance

🏭 Biochemical & Molecular Biology Research – Used in RNA-related enzymatic studies & transcription assays

Preparation & Storage Guidelines

1️⃣ Dissolve in Organic Solvents – Recommended solvents include DMSO, ethanol, methanol (avoid water).

2️⃣ Use Filtration for Sterile Solutions – Do not autoclave as Rifampicin decomposes at high temperatures.

3️⃣ Store at -20°C, Protect from Light & Moisture – Keep tightly sealed to prevent degradation.

4️⃣ Prepare Fresh Solutions When Needed – Rifampicin solutions degrade over time in aqueous environments.

Ordering Information

Regulatory & Safety Information

⚠️ For Research Use Only (RUO). Not intended for clinical or therapeutic applications.

⚠️ Light & Moisture Sensitive – Store in a dry, well-ventilated area under inert gas conditions.

⚠️ Use Protective Equipment (PPE) – Avoid direct skin contact & inhalation during handling.

Why Choose Sigma-Aldrich Rifampicin (≥95%, HPLC)?

🔹 High Purity (≥95% by HPLC) for Reliable Research Applications

🔹 Broad-Spectrum Activity Against Gram-Positive, Gram-Negative & Mycobacteria

🔹 Validated for TB Research, RNA Polymerase Inhibition, & Drug Resistance Studies

🔹 Stable Under Controlled Conditions (-20°C, Light-Protected, Dry Environment)

🔹 Available in Multiple Pack Sizes for Research Flexibility

For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀