Sigma-Aldrich Nystatin Powder, Bioreagent, Suitable For Cell Culture

High-Purity Antifungal Agent for Microbiology, Cell Culture, and Biomedical Research

Sigma-Aldrich Nystatin (BioReagent, Cell Culture Grade) is a polyene macrolide antifungal agent widely used in microbiology, molecular biology, and cell culture applications. It is produced by Streptomyces noursei ATCC 11455 and is effective against fungi, yeasts, and molds by binding to ergosterol in fungal cell membranes, leading to increased permeability and cell death.

This high-purity, endotoxin-tested Nystatin powder is essential for preventing fungal contamination in cell culture, studying membrane cholesterol interactions, and investigating lipid raft-associated cellular functions.

Key Features & Benefits

✅ High-Purity BioReagent-Grade Nystatin – Ensures reproducibility in microbiology & cell culture applications

✅ Broad-Spectrum Antifungal Activity – Effective against yeasts, molds, and fungal pathogens

✅ Validated for Mammalian Cell Culture – Used at 50 mg/L for contamination prevention

✅ Mode of Action: Binds to Ergosterol – Increases membrane permeability, leading to fungal cell death

✅ Lipid Raft Inhibitor – Blocks membrane-associated cholesterol interactions in research studies

✅ Stable in Moderately Alkaline Media & at 37°C for 3 Days – Suitable for long-term experiments

✅ Dissolves in Organic Solvents (Methanol, Ethanol, DMSO) – Ensures flexibility in applications

Technical Specifications

Mechanism of Action & Resistance

Mode of Action:

• Nystatin binds to ergosterol in fungal cell membranes, creating ion-permeable channels that lead to leakage of essential cellular components.
• It also acts as a lipid raft inhibitor, impacting membrane-associated cholesterol interactions.
• Nystatin is widely used for fungal inhibition in mammalian cell cultures and microbiology studies.

Mode of Resistance:

• Fungi resistant to Nystatin alter ergosterol composition, reducing drug binding.
• Some yeasts and molds overexpress efflux pumps, lowering intracellular drug accumulation.

Antimicrobial Spectrum:

✔ Yeasts (Candida spp., Saccharomyces cerevisiae)

✔ Fungal Pathogens (Aspergillus spp., Cryptococcus spp.)

✔ Molds & Dermatophytes

Applications

🔬 Cell Culture Contamination Prevention – Used at 50 mg/L to prevent fungal contamination in mammalian cell cultures

🦠 Antifungal Drug Resistance Studies – Evaluates fungal susceptibility to Nystatin

⚗️ Microbiology & Fungal Pathogen Research – Effective against yeasts, molds, and dermatophytes

🧪 Lipid Raft & Membrane Cholesterol Studies – Investigates cell membrane interactions & lipid microdomains

🏭 Pharmaceutical & Biopharmaceutical Research – Used in antifungal drug formulation & screening

Preparation & Storage Guidelines

1️⃣ Preparation of Stock Solution

• Dissolve 5 mg/mL in DMSO, methanol, ethanol, or formamide.
• Insoluble in water – prepare as a suspension if needed.
• Avoid autoclaving; filter sterilization is recommended.

2️⃣ Recommended Working Concentrations

• Cell Culture Contamination Prevention: 50 mg/L
• Antifungal Susceptibility Testing: Variable (based on CLSI/EUCAST guidelines)

3️⃣ Storage Conditions

• Store at 2–8°C in a dry, well-ventilated area.
• Protect from light & air to prevent degradation.
• Aqueous suspensions degrade quickly; prepare fresh when needed.

Ordering Information

Regulatory & Safety Information

⚠️ For Research Use Only (RUO). Not intended for clinical or therapeutic applications.

⚠️ Hygroscopic & Light-Sensitive – Store in a dry, well-ventilated area.

⚠️ Use Protective Equipment (PPE) – Avoid direct skin contact & inhalation.

Why Choose Sigma-Aldrich Nystatin Powder (BioReagent, Cell Culture Grade)?

🔹 High-Purity Antibiotic (≥4,400 Units per mg) for Reliable Research

🔹 Effective Against Yeasts, Molds, & Fungal Pathogens

🔹 Validated for Mammalian Cell Culture & Antifungal Studies

🔹 Stable Under Proper Storage Conditions (2–8°C)

🔹 Available in Multiple Pack Sizes for Research Flexibility

For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀