Sigma-Aldrich Formamide OmniPur® Grade, >= 99%, Deionized

Molecular Biology Grade Reagent for Nucleic Acid Hybridization and Molecular Assays

Sigma-Aldrich Formamide (OmniPur® Grade) is a molecular biology-grade, ≥99% pure, and deionized reagent ideal for applications that require the destabilization of nucleic acid duplexes, such as nucleic acid hybridization protocols, Northern and Southern blotting, and in situ hybridization. Its high purity and absence of nuclease and protease contaminants make it highly suitable for sensitive molecular biology workflows.

Formamide is commonly used to reduce the melting temperature of nucleic acid duplexes, allowing hybridization reactions to occur at lower, more manageable temperatures, which minimizes sample degradation and improves probe specificity.

Key Features & Benefits

• High Purity (≥99%)
  Verified by gas chromatography (GC), ensuring consistent performance in temperature-sensitive and structure-specific assays.
• Certified Molecular Biology Grade
  Free from DNase, RNase, protease, and phosphatase activity—validated for hybridization applications.
• UV Transparency
  Absorbance values at 260, 270, and 280 nm (≤0.03, ≤0.05, and ≤0.08 AU respectively at 0.5 M in water) ensure minimal UV interference in spectrophotometric assays.
• Deionized and Low Conductivity
  Conductivity ≤100 µmho, supporting compatibility with high-sensitivity molecular detection workflows.
• Suitable for Critical Hybridization Applications
  Widely used in microarray workflows, fluorescent in situ hybridization (FISH), and Northern/Southern blotting.

Applications

• DNA/RNA Hybridization
  Used to lower the Tm (melting temperature) of nucleic acid duplexes during probe binding.
• In Situ Hybridization (ISH and FISH)
  Enhances nucleic acid probe accessibility while preserving sample integrity.
• Microarrays
  Included in hybridization buffer formulations for denaturation and stringency control.
• Southern & Northern Blotting
  Enables high-specificity probe binding at lower hybridization temperatures.

Specifications Table

Available Pack Sizes & SKUs

Sigma-Aldrich Formamide OmniPur® Grade, ≥99%, Deionized is a trusted, high-performance reagent for researchers conducting nucleic acid hybridization, blotting, or probe-based assays. With verified purity, low UV absorbance, and nuclease-free certification, it offers the reliability required for demanding molecular biology protocols.