Sigma-Aldrich Cholecalciferol analytical standard, ≥97.0%

Cholecalciferol, also known as Vitamin D₃, is a high-purity analytical reference standard used for the quantitative and qualitative determination of vitamin D₃ in pharmaceutical, food, and biological samples. Supplied as a crystalline white solid with an assay of ≥97.0%, this product is designed to support accurate, reproducible analytical measurements in advanced chromatographic and mass spectrometric applications.

Vitamin D₃ is a fat-soluble secosteroid that plays a critical role in calcium and phosphorus homeostasis, bone metabolism, and cellular regulation. In analytical and biomedical research, cholecalciferol is widely employed as a calibration and internal standard for vitamin D analysis in serum, oils, and pharmaceutical formulations.

The product is synthetically derived (organic – wool) and is packaged in flame-sealed ampules under argon, ensuring excellent stability and protection from oxidation. Storage at 2–8 °C is recommended to maintain integrity.

Key Features

• High-purity analytical standard for vitamin D₃
• ≥97.0% assay for reliable quantification
• Suitable for HPLC, UHPLC, and LC-ESI-MS/MS analysis
• Crystalline, white solid with defined melting point
• Packaged in argon-sealed ampules for stability
• Suitable for pharmaceutical, food, and clinical research

Applications

• Internal standard for HPLC analysis of serum vitamin D levels
• Quantification of vitamin D₃ in oils using LC-ESI-MS/MS
• Analytical reference standard for pharmaceutical formulations
• Method development and validation for vitamin D analysis
• Food, nutritional, and biochemical research

Biochemical & Physiological Relevance

Vitamin D₃ is obtained through diet or synthesized in the skin upon exposure to ultraviolet radiation. Following absorption, it is stored in adipose tissue and exerts its biological effects via the vitamin D receptor (VDR), a ligand-dependent transcription factor. Activated vitamin D signaling regulates cell proliferation and differentiation, supports bone mineralization, and maintains calcium homeostasis. Deficiency is commonly associated with bone disorders and chronic kidney disease.

Product Information

• Product Name: Cholecalciferol (Vitamin D₃), Analytical Standard
• Synonyms: (+)-Vitamin D₃, Activated 7-dehydrocholesterol, Calciol
• CAS Number: 67-97-0
• Empirical Formula: C₂₇H₄₄O
• Molecular Weight: 384.64
• Form: Crystalline solid
• Color: White
• Assay: ≥97.0%
• Melting Point: 83–86 °C
• Biological Source: Synthetic (organic – wool)
• Storage Temperature: 2–8 °C
• Quality Level: 200
• Packaging: Flame-sealed ampules under argon

Available Pack Sizes

Cholecalciferol (Vitamin D₃) Analytical Standard provides a robust, high-quality reference material for vitamin D analysis across clinical, pharmaceutical, food, and nutritional research. Its certified purity, stable packaging, and broad analytical compatibility make it an essential standard for laboratories requiring precise vitamin D₃ quantification.