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Specifications:
| Application | molecular biology | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Analytical grade | Formula | C₂₄H₂₇N₃O₅ |
Potent LpxC Inhibitor for Gram-Negative Bacterial Lipid A Biosynthesis Studies
CHIR-090 is a high-purity, tight-binding inhibitor of LpxC, a zinc-dependent UDP-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase involved in lipid A biosynthesis—an essential component of gram-negative bacterial outer membranes. Supplied at ≥98% purity (by HPLC), CHIR-090 is widely used in antibacterial research, mode-of-action studies, and LpxC enzyme inhibition assays.
CHIR-090 demonstrates nanomolar-level inhibitory activity against LpxC from Aquifex aeolicus and strong antibiotic properties comparable to ciprofloxacin against strains of Pseudomonas aeruginosa and Escherichia coli. Its ability to inhibit lipid A synthesis makes it an important tool compound for exploring novel antibiotic targets in gram-negative pathogens.
Key Features & Benefits
- ✅ ≥98% Purity (HPLC) – High-quality for biochemical and microbiological assays
- ✅ Potent LpxC Inhibitor – Targets essential lipid A biosynthesis pathway in gram-negative bacteria
- ✅ Nanomolar Binding Affinity – A. aeolicus LpxC Ki = 1.0–1.7 nM
- ✅ Effective at Low Concentrations – 98% inhibition of E. coli and A. aeolicus LpxC at 0.5 µg/mL (1.15 µM)
- ✅ Antibiotic Activity Comparable to Ciprofloxacin – Demonstrated efficacy against P. aeruginosa PAO1 and E. coli strains in disk diffusion tests
- ✅ Ideal for Drug Discovery – Valuable for identifying and validating novel antibiotic targets in resistant gram-negative bacteria
Product Specifications
| Attribute | Details |
|---|---|
| Product Name | CHIR-090 |
| Chemical Purity | ≥98% (HPLC) |
| Target Enzyme | LpxC (zinc-dependent deacetylase) |
| CAS Number | 728865-23-4 |
| Inhibition Data | E. coli & A. aeolicus LpxC: 98% inhibition at 0.5 µg/mL (1.15 µM) |
| Binding Affinity (Ki) | 1.0–1.7 nM (for A. aeolicus LpxC) |
| Form | Solid (refer to technical sheet for solubility) |
| Applications | Antibacterial research, enzyme inhibition, lipid A biosynthesis studies |
Applications
🧫 Antibiotic Mode-of-Action Studies – Probe LpxC function in gram-negative bacterial lipid A pathways
🔬 Target Validation for Drug Development – Test novel antibacterial candidates using CHIR-090 as a benchmark
🧪 High-Throughput Screening – Use in in vitro assays to identify LpxC inhibitors
💊 Comparative Antibiotic Testing – Compare efficacy to known antibiotics like ciprofloxacin
🧬 Gram-Negative Bacterial Research – Study essential biosynthetic processes and membrane integrity
Sigma-Aldrich CHIR-090 ≥98% (HPLC) is a high-potency biochemical tool for targeted inhibition of LpxC, supporting critical antibiotic resistance research. With its demonstrated efficacy against clinically relevant gram-negative bacteria, CHIR-090 is essential for scientists developing next-generation antibacterial agents.
- Pack Size: 5mg 25mg