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Virplex® Gastrobacteria RealTime PCR Kit, 10-Plex, 48 Rxn
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Specifications:
| Application | qPCR, Molecular Diagnostics | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Lyophilized |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Virplex® Gastrobacteria RealTime PCR Kit is a multiplex real-time PCR assay designed for the detection of nucleic acids from major gastrointestinal bacterial pathogens in human stool samples. This CE-IVDR certified kit provides laboratories with a comprehensive diagnostic solution for diarrheal diseases, ensuring rapid, sensitive, and reliable detection of multiple pathogens in a single reaction.
Key Features
- Broad pathogen coverage – simultaneous detection of Salmonella spp., Yersinia enterocolitica, Campylobacter coli/jejuni, Campylobacter spp., Vibrio spp., Aeromonas spp., Clostridioides difficile toxin B, enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), enteroinvasive E. coli (EIEC), and Shigella spp.
- Multiplex design – multiple bacterial targets in a single reaction, reducing turnaround time.
- Endogenous internal control – monitors amplification inhibition, nucleic acid integrity, and set-up accuracy.
- Lyophilized reagents – master mix and positive control provided in lyophilized format for long-term stability and reduced transportation costs.
- User-friendly workflow – simple extraction using included swabs and dilution tubes minimizes PCR inhibitors.
- Harmonized PCR protocol – same amplification program as Virplex® Gastrovirus and Gastroparasites 1 RealTime PCR Kits, allowing up to 18 targets per reaction.
- CE-IVDR certified – compliant with European diagnostic regulations.
Applications
- Molecular diagnosis of bacterial gastrointestinal infections.
- Rapid detection of diarrheal pathogens in stool samples.
- Syndromic testing workflows for gastroenteritis.
- Epidemiological surveillance and outbreak investigation.
- Clinical diagnostics in hospital and public health laboratories.
Product Details
| Property | Value |
|---|---|
| Product Name | Virplex® Gastrobacteria RealTime PCR Kit |
| Reference | RTPCR029-LPD |
| Nº Tests | 48 |
| Class | CE-IVDR (CE0123) |
| Format | Real-time multiplex PCR kit |
| Specimen Type | Human stool samples |
| Pathogens Detected | Salmonella spp., Yersinia enterocolitica, Campylobacter coli/jejuni, Campylobacter spp., Vibrio spp., Aeromonas spp., Clostridioides difficile toxin B, ETEC, STEC, EIEC, Shigella spp. |
| Internal Control | Endogenous control for inhibition and integrity |
| Reagents | Lyophilized master mix and positive control |
| Workflow | Compatible with Gastrovirus and Gastroparasites 1 kits (shared PCR protocol) |
Combined Target Panel (18 Targets)
| Category | Target Organism / Gene | Clinical Relevance |
|---|---|---|
| Bacterial Pathogens (Gastrobacteria Kit) | Salmonella spp. | Major cause of bacterial gastroenteritis and foodborne illness |
| Yersinia enterocolitica | Enteric fever, acute gastroenteritis, post-infectious complications | |
| Campylobacter coli/jejuni | Leading cause of bacterial diarrhea, linked to Guillain-Barré syndrome | |
| Campylobacter spp. (other species) | Gastroenteritis and enteritis | |
| Vibrio spp. | Cholera (V. cholerae), gastroenteritis from seafood (V. parahaemolyticus, V. vulnificus) | |
| Aeromonas spp. | Gastroenteritis, foodborne diarrhea | |
| Clostridioides difficile toxin B | Antibiotic-associated diarrhea, pseudomembranous colitis | |
| Enterotoxigenic Escherichia coli (ETEC) | Traveler’s diarrhea, watery gastroenteritis | |
| Shiga toxin-producing E. coli (STEC) | Hemorrhagic colitis, HUS (Hemolytic Uremic Syndrome) | |
| Enteroinvasive E. coli (EIEC) | Dysentery-like diarrhea | |
| Shigella spp. | Bacillary dysentery |
The Virplex® Gastrobacteria RealTime PCR Kit provides laboratories with a comprehensive, reliable, and efficient tool for the diagnosis of gastrointestinal bacterial infections. With broad pathogen coverage, lyophilized reagents, and a streamlined workflow, it ensures accuracy, reproducibility, and compliance in clinical diagnostics and public health surveillance.