Remel™ BactiDrop™ Spot Indole, 0.75 mL, 50/pk

The Thermo Scientific™ Remel™ BactiDrop™ Spot Indole is a reliable reagent for determining the ability of microorganisms to hydrolyze tryptophan and produce indole. This rapid, easy-to-use spot test reagent provides quick and accurate results, making it a valuable tool in microbiological diagnostics for differentiating bacterial species such as Proteus spp. and Escherichia coli.

Key Features:

1. Ready-to-Use:
   • Pre-filled ampules with 1% w/v ρ-dimethylaminocinnamaldehyde (DMACA) for immediate application.
2. Fast Results:
   • Detects indole production within 1–3 minutes, enabling rapid identification.
3. Easy Interpretation:
   • Positive results yield a blue-green color, while negative results are pink, making it easy to differentiate.
4. High Sensitivity:
   • Detects as little as 3 mcg of indole per mL, ensuring precise and reliable performance.
5. Compact and Convenient:
   • Each box contains 50 x 0.75 mL ampules, ensuring sufficient reagent for multiple tests.

Principle of the Test:

• Tryptophanase Enzyme:
  • Tryptophanases in microorganisms hydrolyze tryptophan to produce indole.
  • Indole reacts with DMACA in the reagent to form a blue-green condensation product.
• Result Interpretation:
  • Positive: Blue-green color (indole produced).
  • Negative: Pink color (no indole production).

Applications:

1. Clinical Microbiology:
   • Used to differentiate Proteus spp., Escherichia coli, and other bacteria based on indole production.
2. Research:
   • Supports studies on microbial metabolism and enzymatic activity.
3. Quality Control:
   • Applicable in routine bacterial identification workflows in clinical and industrial microbiology labs.

Specifications:

Advantages:

1. Rapid and Reliable:
   • Provides quick results, minimizing waiting time in diagnostic workflows.
2. High Sensitivity:
   • Detects even low levels of indole production for accurate microbial identification.
3. Ease of Use:
   • Eliminates preparation steps with pre-filled ampules for direct application.
4. Cost-Effective:
   • Each ampule supports multiple tests, reducing reagent waste.

Usage Instructions:

1. Place a drop of BactiDrop Spot Indole reagent directly onto a colony grown on appropriate agar.
2. Observe the reaction for 1–3 minutes.
3. Interpret results based on the color change:
   • Blue-green: Positive for indole production.
   • Pink: Negative for indole production.

Storage and Handling:

• Store ampules at 20°C to 25°C in a dry environment.
• Use before the expiration date indicated on the packaging.

Intended Use:

The BactiDrop Spot Indole reagent is designed for professional laboratory use to detect the ability of microorganisms to split indole from tryptophan. This test is not automated and is part of diagnostic workflows aiding in bacterial differentiation.

References:

1. Vracko, R. and J.C. Sherris. 1963. Am. J. Clin. Path. 39:429-432.
2. Lowrance, B.L., P. Reich, and W.H. Traub. 1969. Appl. Microbiol. 17:923-924.

Product Details:

The Thermo Scientific™ Remel™ BactiDrop™ Spot Indole is an essential reagent for rapid, sensitive, and reliable identification of indole-producing microorganisms. Its ease of use, fast results, and high accuracy make it indispensable in clinical and research microbiology laboratories.