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Thermo Scientific™ Glycogen, molecular biology grade, 2 x 0.25 mL
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | Formula | C₂₄H₄₂O₂₁ |
High-Efficiency Carrier for Nucleic Acid Precipitation in Molecular Biology Applications
Thermo Scientific™ Glycogen (Molecular Biology Grade) is a highly purified, inert polysaccharide derived from oysters, designed to significantly enhance the recovery of nucleic acids—including low-abundance DNA and RNA—during alcohol precipitation. Its strong precipitation-enhancing capability makes it an ideal choice for workflows involving dilute or low-yield nucleic acid samples, such as those encountered in cloning, PCR, or sequencing protocols.
Unlike other carriers such as tRNA or polyacrylamide, glycogen forms a clearly visible pellet, simplifying recovery and reducing sample loss. It is non-inhibitory to enzymatic reactions and compatible with common downstream processes like PCR, ligation, DNA sequencing, and in vitro transcription.
Key Features & Benefits
- High-Efficiency Nucleic Acid Carrier – Recovers DNA/RNA from as little as 20 pg or oligonucleotides longer than 8 bases
- Visible Pellet Formation – Glycogen forms a precipitate upon ethanol addition, enhancing pellet visibility during centrifugation
- Non-Interfering – Inert carrier that does not affect enzymatic reactions or spectrophotometric readings (A260/280)
- Molecular Biology Grade – Optimized for DNA recovery, suitable for cloning, transformation, PCR, and more
- Superior to tRNA or Polyacrylamide – Offers higher recovery efficiency in ethanol precipitation protocols
Applications
- Ethanol or Isopropanol Precipitation of DNA – Improves recovery from low-concentration or dilute samples
- RNA Purification and Cleanup – For DNA-grade glycogen use only (#R0561); RNA-grade available separately (#R0551)
- Cloning & PCR Workflows – Recovers low-concentration PCR products or cloning fragments
- DNA/RNA Labeling & Enzymatic Reactions – Compatible with ligation, endonuclease digestion, cDNA synthesis
- Bacterial Transformation & In Vitro Transfection – Enhances nucleic acid handling with no adverse effects
Specifications
| Attribute | Specification |
|---|---|
| Product Name | Glycogen, Molecular Biology Grade |
| Catalog Number | R0561 |
| Form | Liquid |
| Chemical Type | Glycogen (polysaccharide from oyster source) |
| Quantity | 2 × 0.25 mL |
| Recommended Use | DNA precipitation workflows |
| Enzymatic Compatibility | Non-inhibitory up to 8 μg/μL |
| Pellet Visibility | Forms clearly visible precipitate |
| Storage Conditions | Store refrigerated (2–8°C) |
| Regulatory Use | For Research Use Only |
Thermo Scientific™ Glycogen, Molecular Biology Grade is the ideal nucleic acid carrier for researchers requiring maximum DNA recovery from low-yield or dilute samples. With high purity, excellent pellet visibility, and compatibility with enzymatic workflows, it supports a wide range of molecular biology and genetic analysis applications.