Protamex® Protease from Bacillus sp., >1.5 AU-N/g

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Specifications:
Application pharmaceutical, Protein Biology
Storage Temperature 2-8°C
Product Type Enzymes Forms Powder
Product Brand Sigma-Aldrich
Product Grade Analytical grade

Versatile Broad-Range Protease for Food, Pharmaceutical, and Industrial Applications

Protamex® Protease is a broad-spectrum endopeptidase preparation derived from Bacillus species, offering high enzymatic activity of >1.5 AU-N/g. This microbial protease is extensively used in a variety of industries including food and beverage, pharmaceuticals, biotechnology, leather, and waste treatment. Its broad pH stability (pH 5–11) and optimal activity at pH 8.0 and 60°C make it a highly effective enzyme for protein hydrolysis, enzymatic digestion, and bioprocessing applications.

The enzyme maintains full activity at pH 8.0 and up to 95% residual activity at 60°C, making it ideal for high-yield industrial processes and reliable performance in proteolytic digestion workflows.

Key Features & Benefits

  • High Activity: Minimum proteolytic activity of >1.5 AU-N/g
  • Broad pH Range (5.0–11.0): Effective in a wide variety of processing environments
  • Stable at Elevated Temperatures: Maintains up to 95% activity at 60°C
  • Derived from Bacillus sp.: Trusted microbial source with wide industrial adoption
  • Available in Two Convenient Pack Sizes: 50 g and 250 g options for research and scale-up

🧪 Applications

  • Protein Hydrolysate Production – Widely used in food-grade hydrolysis of plant or animal proteins
  • Pharmaceutical Enzyme Systems – Evaluating protease inhibitor activity or controlled protein digestion
  • Bioprocessing & Waste Management – Enhancing organic breakdown and recovery in industrial effluent
  • Leather Industry – Pre-treatment and enzymatic dehairing of hides
  • Biosensor Research – Detecting protease biomarkers from microbial or biological systems

🔧 Technical Specifications

AttributeSpecification
Product NameProtamex® Protease from Bacillus sp.
Enzyme Activity>1.5 AU-N/g solid
Source OrganismBacillus species
FormSolid powder
pH Activity Range5.0 – 11.0
Optimal pH8.0
Optimal Temperature60°C
Thermal Stability95% activity retained at 60°C
SolubilitySoluble in aqueous buffers
Pack Sizes50 g (P0029-50G), 250 g (P0029-250G)
StorageStore in a cool, dry place; reseal after use
Regulatory UseFor research and industrial processing use only

📚 Scientific References & Applications

  • Protease Inhibitor Evaluation – Used to test inhibition activity in enzyme-based assays
  • Batch Fermentation Optimization – Applied in high-yield microbial fermentation systems
  • Biosensor Development – Detection of Bacillus licheniformis protease biomarkers
  • Food Industry – Production of digestible peptides and bioactive compounds from protein sources

Protamex® Protease from Bacillus sp. offers a high-performance, versatile enzymatic solution for industries requiring efficient protein hydrolysis, from food production to pharmaceutical development. With its broad pH and temperature stability, high purity, and reliable enzymatic activity, it supports a wide spectrum of research, bioprocessing, and industrial applications.

  • Pack Size:   250g 50g
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