Invitrogen™ Power Blotter Pre-cut Membranes and Filters, PVDF mini, 0.45 μm, 7 x 8.4 cm, 20 Blots

The Invitrogen™ Power Blotter Pre-cut Membranes and Filters, PVDF, Mini are ready-to-use membrane and filter paper stacks designed for efficient protein transfer from mini-size gels using the Power Blotter System. These pre-cut membranes and filters eliminate manual cutting, reducing variability and contamination risks, ensuring high-quality and reproducible Western blot results.

Each stack includes 20 sheets of polyvinylidene difluoride (PVDF) membrane (0.45 μm pore size, low fluorescence) and 80 sheets of absorbent blotting paper (0.85 mm thickness), optimized for high-efficiency semi-dry protein transfer in less than 10 minutes.

Key Features & Benefits

1. Ready-to-Use for Fast & Reliable Protein Transfer

• Pre-cut membranes and filters minimize preparation time and reduce errors.
• Designed for mini-size gels (8 cm × 8 cm), eliminating the need for trimming.
• Optimized for use with Power Blotter 1-Step Transfer Buffer for high-efficiency blotting.

2. High-Performance PVDF Membrane (0.45 μm Pore Size, Low Fluorescence)

• Higher binding capacity than nitrocellulose (240 μg/cm²).
• Pre-activated and ready to use, eliminating the need for alcohol pretreatment.
• Compatible with staining, immunodetection, fluorescence, and radiolabeling detection methods.
• Provides low background levels and increased sensitivity for fluorescent probing compared to regular PVDF or nitrocellulose membranes.

3. Reliable & Reproducible Protein Transfer

• Uniform membrane thickness and high surface area ensure even protein binding.
• Validated for Power Blotter System, providing consistent and reproducible results.
• Compatible with commonly used Western blot detection methods.

4. Mini-Size Format for Maximum Compatibility

• Dimensions: 7 cm × 8.4 cm, compatible with:
  • Invitrogen Novex, Bolt, and NuPAGE mini gels.
  • Standard precast or homemade mini gels.
• Easily fits into standard Western blot workflows, improving efficiency.

Specifications

Kit Components & Storage

Storage Conditions:

• Store at room temperature in a dry environment.
• Handle membranes with gloves to prevent contamination.
• Keep away from moisture and direct sunlight for optimal performance.

Available Product Variants

Compatible Accessories & Consumables

Applications

1. Western Blotting & Protein Analysis

• Rapid protein transfer for immunodetection and proteomics research.
• Optimized for PVDF-based Western blot detection methods.
• Superior retention of low-abundance and hydrophobic proteins.

2. Molecular Biology & Biochemistry Research

• Designed for multi-gel transfer workflows, improving research efficiency.
• Supports high-sensitivity detection using chemiluminescence, fluorescence, and radiolabeling methods.

3. Biotech & Pharmaceutical Research

• Used for protein expression studies, immunoblotting, and biotherapeutic research.
• High-throughput compatibility with Power Blotter Systems for large-scale experiments.

Storage & Handling

• Store at room temperature in a dry environment.
• Handle with gloves to prevent contamination of PVDF membranes.
• Ensure proper alignment with gels for optimal transfer efficiency.

Ordering Information

The Invitrogen™ Power Blotter Pre-cut Membranes and Filters, PVDF, Mini provide fast, efficient, and high-sensitivity semi-dry Western blot protein transfers. Designed for use with Power Blotter Systems, these pre-assembled transfer stacks streamline the blotting process, offering high-protein binding capacity and uniform transfer performance.