Invitrogen™ Power Blotter Pre-cut Membranes and Filters, nitrocellulose, 0.45 μm, mini, 7 x 8.4 cm, 20 Blots
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
Application | Protein Electrophoresis & Western Blotting | ||
Storage Temperature | Room Temperature | ||
Product Type | Membranes | Forms | Kit with Various components |
Product Brand | Thermo Fisher Scientific™ | ||
Product Grade | Molecular Biology | ||
The Invitrogen™ Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Mini are ready-to-use membrane and filter paper stacks designed for efficient protein transfer from mini-size gels using the Power Blotter System. These pre-cut membranes and filters eliminate manual cutting, reducing variability and contamination risks, ensuring high-quality and reproducible Western blot results.
Each stack includes 20 sheets of pure nitrocellulose membrane (0.45 μm pore size) and 80 sheets of absorbent blotting paper (0.85 mm thickness), optimized for high-efficiency semi-dry protein transfer in less than 10 minutes.
Key Features & Benefits
1. Ready-to-Use for Fast & Reliable Protein Transfer
- Pre-cut membranes and filters minimize preparation time and reduce errors.
- Designed for mini-size gels (8 cm × 8 cm), eliminating the need for trimming.
- Optimized for use with Power Blotter 1-Step Transfer Buffer for high-efficiency blotting.
2. High-Performance Nitrocellulose Membrane (0.45 μm Pore Size)
- Composed of 100% pure nitrocellulose for high protein binding efficiency (209 μg/cm²).
- Excellent signal retention for chemiluminescence, chromogenic, and fluorescence-based detection.
- Provides low background and easy blocking, improving Western blot sensitivity.
3. Reliable & Reproducible Protein Transfer
- Uniform membrane thickness and high surface area ensure even protein binding.
- Validated for Power Blotter System, providing consistent and reproducible results.
- Compatible with commonly used Western blot detection methods.
4. Mini-Size Format for Maximum Compatibility
- Dimensions: 7 cm × 8.4 cm, compatible with:
- Invitrogen Novex, Bolt, and NuPAGE mini gels.
- Standard precast or homemade mini gels.
- Easily fits into standard Western blot workflows, improving efficiency.
Specifications
Property | Details |
---|---|
Model | Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Mini |
Mode of Transfer | Semi-Dry |
Membrane Material | Nitrocellulose |
Pore Size | 0.45 μm |
Protein Binding Capacity | 209 μg/cm² |
Transfer Time | Less than 10 minutes |
Compatible Gels | Novex, Bolt, NuPAGE mini gels |
Dimensions (L × W) | 7 cm × 8.4 cm |
Thickness | 0.85 mm |
Shipping Condition | Room Temperature |
Sufficient For | 20 blots |
Unit Size | Each |
Kit Components & Storage
Component | Description |
---|---|
Pre-cut Nitrocellulose Membranes | 20 sheets, 0.45 μm pore size, mini-size (7 cm × 8.4 cm) |
Absorbent Blotting Paper | 80 sheets, 0.85 mm thickness |
Storage Conditions:
- Store at room temperature in a dry environment.
- Handle membranes with gloves to prevent contamination.
- Keep away from moisture and direct sunlight for optimal performance.
Available Product Variants
Catalog Number | Sufficient For |
---|---|
PB7220 | 20 Blots |
Compatible Accessories & Consumables
Product | Description | Quantity |
---|---|---|
Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Regular Size | Nitrocellulose membranes and filters for midi gels | 20 stacks |
Power Blotter Pre-cut Membranes and Filters, PVDF, Mini | PVDF membrane transfer stacks for semi-dry blotting | 20 stacks |
Power Blotter Pre-cut Membranes and Filters, PVDF, Regular Size | Larger PVDF transfer stacks | 20 stacks |
Power Blotter 1-Step Transfer Buffer | Optimized buffer for semi-dry Western blotting | 1L |
Power Blotter Station | Integrated blotting system for protein transfer | 1 unit |
Applications
1. Western Blotting & Protein Analysis
- Efficient protein transfer for immunodetection and biochemical research.
- Optimized for nitrocellulose-based Western blot detection methods.
- Ensures high-quality, reproducible protein blotting results.
2. Molecular Biology & Biochemistry Research
- Designed for high-throughput Western blotting workflows.
- Supports high-sensitivity detection using chemiluminescence, fluorescence, and staining methods.
3. Biotech & Pharmaceutical Research
- Used for protein expression studies, immunoblotting, and biotherapeutic research.
- High-throughput compatibility with Power Blotter Systems for large-scale experiments.
Storage & Handling
- Store at room temperature in a dry environment.
- Handle with gloves to prevent contamination of transfer membranes.
- Ensure proper alignment with gels for optimal transfer efficiency.
Ordering Information
Catalog Number | Description | Sufficient For |
---|---|---|
PB7220 | Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Mini | 20 Blots |
The Invitrogen™ Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Mini provide fast, efficient, and high-sensitivity semi-dry Western blot protein transfers. Designed for use with Power Blotter Systems, these pre-assembled transfer stacks streamline the blotting process, offering high-protein binding capacity and uniform transfer performance.