Invitrogen™ Power Blotter Pre-cut Membranes and Filters, nitrocellulose, 0.45 μm, mini, 7 x 8.4 cm, 20 Blots

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    Specifications:
    Application Protein Electrophoresis & Western Blotting
    Storage Temperature Room Temperature
    Product Type Membranes Forms Kit with Various components
    Product Brand Thermo Fisher Scientific™
    Product Grade Molecular Biology

    The Invitrogen™ Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Mini are ready-to-use membrane and filter paper stacks designed for efficient protein transfer from mini-size gels using the Power Blotter System. These pre-cut membranes and filters eliminate manual cutting, reducing variability and contamination risks, ensuring high-quality and reproducible Western blot results.

    Each stack includes 20 sheets of pure nitrocellulose membrane (0.45 μm pore size) and 80 sheets of absorbent blotting paper (0.85 mm thickness), optimized for high-efficiency semi-dry protein transfer in less than 10 minutes.

    Key Features & Benefits

    1. Ready-to-Use for Fast & Reliable Protein Transfer

    • Pre-cut membranes and filters minimize preparation time and reduce errors.
    • Designed for mini-size gels (8 cm × 8 cm), eliminating the need for trimming.
    • Optimized for use with Power Blotter 1-Step Transfer Buffer for high-efficiency blotting.

    2. High-Performance Nitrocellulose Membrane (0.45 μm Pore Size)

    • Composed of 100% pure nitrocellulose for high protein binding efficiency (209 μg/cm²).
    • Excellent signal retention for chemiluminescence, chromogenic, and fluorescence-based detection.
    • Provides low background and easy blocking, improving Western blot sensitivity.

    3. Reliable & Reproducible Protein Transfer

    • Uniform membrane thickness and high surface area ensure even protein binding.
    • Validated for Power Blotter System, providing consistent and reproducible results.
    • Compatible with commonly used Western blot detection methods.

    4. Mini-Size Format for Maximum Compatibility

    • Dimensions: 7 cm × 8.4 cm, compatible with:
      • Invitrogen Novex, Bolt, and NuPAGE mini gels.
      • Standard precast or homemade mini gels.
    • Easily fits into standard Western blot workflows, improving efficiency.

    Specifications

    PropertyDetails
    ModelPower Blotter Pre-cut Membranes and Filters, Nitrocellulose, Mini
    Mode of TransferSemi-Dry
    Membrane MaterialNitrocellulose
    Pore Size0.45 μm
    Protein Binding Capacity209 μg/cm²
    Transfer TimeLess than 10 minutes
    Compatible GelsNovex, Bolt, NuPAGE mini gels
    Dimensions (L × W)7 cm × 8.4 cm
    Thickness0.85 mm
    Shipping ConditionRoom Temperature
    Sufficient For20 blots
    Unit SizeEach

    Kit Components & Storage

    ComponentDescription
    Pre-cut Nitrocellulose Membranes20 sheets, 0.45 μm pore size, mini-size (7 cm × 8.4 cm)
    Absorbent Blotting Paper80 sheets, 0.85 mm thickness

    Storage Conditions:

    • Store at room temperature in a dry environment.
    • Handle membranes with gloves to prevent contamination.
    • Keep away from moisture and direct sunlight for optimal performance.

    Available Product Variants

    Catalog NumberSufficient For
    PB722020 Blots

    Compatible Accessories & Consumables

    ProductDescriptionQuantity
    Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Regular SizeNitrocellulose membranes and filters for midi gels20 stacks
    Power Blotter Pre-cut Membranes and Filters, PVDF, MiniPVDF membrane transfer stacks for semi-dry blotting20 stacks
    Power Blotter Pre-cut Membranes and Filters, PVDF, Regular SizeLarger PVDF transfer stacks20 stacks
    Power Blotter 1-Step Transfer BufferOptimized buffer for semi-dry Western blotting1L
    Power Blotter StationIntegrated blotting system for protein transfer1 unit

    Applications

    1. Western Blotting & Protein Analysis

    • Efficient protein transfer for immunodetection and biochemical research.
    • Optimized for nitrocellulose-based Western blot detection methods.
    • Ensures high-quality, reproducible protein blotting results.

    2. Molecular Biology & Biochemistry Research

    • Designed for high-throughput Western blotting workflows.
    • Supports high-sensitivity detection using chemiluminescence, fluorescence, and staining methods.

    3. Biotech & Pharmaceutical Research

    • Used for protein expression studies, immunoblotting, and biotherapeutic research.
    • High-throughput compatibility with Power Blotter Systems for large-scale experiments.

    Storage & Handling

    • Store at room temperature in a dry environment.
    • Handle with gloves to prevent contamination of transfer membranes.
    • Ensure proper alignment with gels for optimal transfer efficiency.

    Ordering Information

    Catalog NumberDescriptionSufficient For
    PB7220Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Mini20 Blots

    The Invitrogen™ Power Blotter Pre-cut Membranes and Filters, Nitrocellulose, Mini provide fast, efficient, and high-sensitivity semi-dry Western blot protein transfers. Designed for use with Power Blotter Systems, these pre-assembled transfer stacks streamline the blotting process, offering high-protein binding capacity and uniform transfer performance.

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