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PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa
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Dye-stained proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1mM NaN3 and 33% glycerol.
Specifications:
| Application | Protein Electrophoresis & Western Blotting |
| Storage Temperature | -20°C |
| Product Type | Protein Gels |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
Thermo Scientific™ PageRuler Plus Prestained Protein Ladder is a ready-to-use protein size standard for SDS-PAGE and western blotting. It contains nine prestained proteins ranging from 10 to 250 kDa, stained in three distinct colors (blue, orange, and green) for easy visualization.
Features:
- Molecular Weight Range: 10 to 250 kDa.
- Color-Coded Bands:
- Blue: Major markers
- Orange: 70 kDa
- Green: 25 kDa
- Convenience: Ready-to-load format eliminates the need for heating, reduction, or adding sample buffer.
- Stability: Supplied in a stable storage buffer to ensure long-term use and consistency.
- Applications:
- Monitor protein migration during SDS-PAGE.
- Assess protein transfer efficiency in western blotting.
- Estimate molecular weights of proteins in gels and membranes.
Applications:
- SDS-PAGE: Track protein migration in polyacrylamide gels.
- Western Blotting: Visualize protein transfer onto membranes.
- Protein Sizing: Accurately estimate molecular weights of target proteins.
Specifications:
| Property | Details |
|---|---|
| Size Range | 10 to 250 kDa |
| Number of Markers | 9 |
| Color Indicators | Blue, Orange, Green |
| Detection Methods | Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm) |
| Gel Compatibility | Bolt™ Bis-Tris Plus, Novex™ Tricine, Novex™ Tris-Glycine, NuPAGE™ Bis-Tris, NuPAGE™ Tris-Acetate, SDS-PAGE |
| Product Line | PageRuler™ |
| Storage Conditions | Store at -20°C |
| Shipping Conditions | Approved for shipment on Wet or Dry Ice |
| Storage Buffer Composition | 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol |
| System Type | Western Blotting, SDS-PAGE |
| Unit Sizes | - 26620: 10 x 250 µL vials - 26619: 2 x 250 µL vials |
Contents & Storage:
- Contents: Prepackaged vials containing prestained protein ladder ready for direct use.
- Storage Buffer: Ensures stability for accurate and reproducible results.
- Shelf Life: Maintain at -20°C to preserve protein integrity.
Use Cases:
- Research Laboratories:
- Protein electrophoresis and western blotting in molecular biology.
- Clinical Research:
- Analyze protein expression in experimental setups.
- Educational Institutions:
- Teaching SDS-PAGE and western blot techniques.
- Pharmaceutical Development:
- Monitor protein purity during drug development.
This product is ideal for researchers requiring reliable, high-quality size markers for protein electrophoresis and blotting workflows.
A comparison between the Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder and the Bio-Rad Precision Plus Protein™ Dual Color Standards:
| Feature | Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder | Bio-Rad Precision Plus Protein™ Dual Color Standards |
|---|---|---|
| Catalog Numbers | 26620 (10 x 250 µL), 26619 (2 x 250 µL) | 1610374 (500 µL), 1610374S (50 µL), 1610394 (2.5 mL) |
| Number of Bands | 9 | 10 |
| Molecular Weight Range | 10 to 250 kDa | 10 to 250 kDa |
| Band Colors | Blue, Orange (70 kDa), Green (25 kDa) | 8 Blue, 2 Pink (25 and 75 kDa) |
| Ready-to-Use Format | Yes | Yes |
| Detection Methods | Colorimetric, NIR Fluorescence (700 nm), RGB Fluorescence (555 nm) | Not specified |
| Recommended Loading Volume | 5 µL (mini gels), 10 µL (large gels) | 10 µL (electrophoresis), 7 µL (blotting) |
| Storage Conditions | -20°C | 6 months at 4°C or –20°C until expiration date |
| Shelf Life | Not specified | 6 months at 4°C or –20°C until expiration date |
| Shipping Conditions | Approved for shipment on Wet or Dry Ice | Shipped at room temperature |
| Applications | SDS-PAGE, Western Blotting | SDS-PAGE, Western Blotting |
Both ladders offer a range of prestained proteins suitable for monitoring protein migration and estimating molecular weights during SDS-PAGE and Western blotting. The choice between them may depend on specific experimental requirements, such as preferred band colors or detection methods.
- Pack Size: 2 x 250 µL 10 x 250 µL