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Invitrogen™ LC3B Polyclonal Antibody, DyLight™ 488, 100 µL
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Autophagy Marker for Fluorescence-Based Detection and Imaging
Specifications:
| Application | Western Blot, immunocytochemistry, Immunohistochemistry, Immunoprecipitation (IP), Immunomicroscopy (IM) | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Secondary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ LC3B Polyclonal Antibody, DyLight™ 488 is a high-quality fluorescently labeled antibody designed for the detection of LC3B (Microtubule-associated protein 1 light chain 3B), a widely recognized marker of autophagy. Conjugated with DyLight™ 488, this antibody enables direct fluorescence-based detection, eliminating the need for secondary antibodies and simplifying experimental workflows.
LC3B plays a critical role in autophagosome formation and is extensively used in studies related to cellular degradation pathways, cancer biology, neurodegeneration, metabolic disorders, and aging.
Key Features
-
Direct Fluorescent Detection (DyLight™ 488)
Enables sensitive detection with excitation/emission maxima at 492/519 nm. -
Polyclonal Antibody (Rabbit IgG)
Provides strong signal intensity through recognition of multiple epitopes. -
Validated for Multiple Applications
Suitable for ICC/IF, Western blot, flow cytometry, and immunohistochemistry. -
Broad Species Reactivity
Cross-reactive with multiple species including human, mouse, rat, and others. -
High Purity (Affinity Purified)
Ensures specificity and reduced background signal. -
Ready-to-Use Format
Eliminates need for secondary antibody labeling steps.
Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 – 1:3,000 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:2,000 |
| Immunohistochemistry (Paraffin/Frozen/Free Floating) | Assay-dependent |
| Flow Cytometry | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
| Immunomicroscopy (IM) | Assay-dependent |
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | LC3B Polyclonal Antibody |
| Brand | Invitrogen™ |
| Catalog Number | PA5-22938 |
| Format | Liquid |
| Conjugate | DyLight™ 488 |
| Excitation/Emission | 492 / 519 nm |
| Host / Isotype | Rabbit / IgG |
| Antibody Type | Polyclonal |
| Immunogen | Synthetic peptide (N-terminal region of human LC3B) |
| Concentration | 0.82 mg/mL |
| Amount | 82 µg |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Preservative | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic), wet ice (international) |
| RRID | AB_11153644 |
Species Reactivity
- Human
- Mouse
- Rat
- Bovine
- Dog
- Pig
- Non-human primate
- Zebrafish
- Yeast
- Bacteria
Target Information
LC3B (MAP1LC3B) is a key protein involved in autophagy, a cellular process responsible for the degradation and recycling of cytoplasmic components. During autophagy, LC3B undergoes post-translational modification and becomes associated with autophagosomal membranes, making it a reliable marker for monitoring autophagic activity.
LC3B is implicated in:
- Cancer progression and therapy response
- Neurodegenerative diseases
- Metabolic disorders
- Aging and cellular stress responses
Application Insight
This antibody has been successfully used in studies investigating diabetic neuropathy, demonstrating its utility in assessing treatment effects on nerve tissue under metabolic stress conditions.
The Invitrogen™ LC3B Polyclonal Antibody, DyLight™ 488 provides a robust and efficient solution for fluorescence-based detection of autophagy. Its direct labeling, broad application compatibility, and high specificity make it an essential tool for researchers studying cellular processes, disease mechanisms, and therapeutic responses.