ONT SpotOn MinION flow cell (R10.4.1)

The SpotOn MinION Flow Cell (R10.4.1) is a consumable designed for use with MinION and GridION devices. It is optimized for high consensus accuracy, particularly when paired with V14 sequencing kits. The R10.4.1 nanopores feature a double reader-head, enabling accurate base calling with a modal raw read accuracy of over 99%.

Key Features

1. High Accuracy with Double Reader-Head:
   • R10.4.1 nanopores enhance sequencing accuracy, ideal for high-consensus applications.
   • Paired with Kit 14 chemistry, it provides modal raw read accuracies above 99%.
2. Extended Usage:
   • Can run continuously for ~72 hours under optimal conditions.
   • Allows for run-stop-wash workflows to reuse the flow cell for multiple libraries.
3. Real-Time Data Streaming:
   • Sequencing data is streamed in real time, enabling users to monitor progress and terminate runs as soon as sufficient data is collected.
4. Multiplexing Capability:
   • Compatible with ONT's barcoding kits, allowing up to 96 samples per run for cost-efficient sequencing.
5. Environmentally Conscious Design:
   • Includes a light shield for optimal performance.
   • Packaging doubles as a returns kit for responsible recycling.

Workflow

1. Preparation:
   • Load the prepared DNA/RNA library onto the flow cell.
   • Install the light shield and start the sequencing experiment.
2. Sequencing:
   • DNA is sequenced by the nanopores, and data is streamed in real time.
   • Use MinKNOW software to monitor performance and analyze sequencing data.
3. Flow Cell Checks:
   • Perform a flow cell check before loading samples to verify the number of active nanopores.
   • Flow cells with fewer than 800 nanopores (reported within 2 days of check) are eligible for replacement.
4. Reuse:
   • Use the Flow Cell Wash Kit to wash out libraries and prepare the flow cell for subsequent runs.

Storage and Stability

• Room Temperature: Store unopened flow cells at room temperature for up to 1 month.
• Refrigerated (2–8°C): Store unopened flow cells for up to 12 weeks.
• Warranty: 12 weeks from receipt; a flow cell check is required within this period.

Compatibility

Kits:

Compatible with all V14 kits, including:

• Ligation Kits: SQK-LSK114, SQK-LSK114-XL.
• Rapid Kits: SQK-RAD114, SQK-RBK114.24, SQK-RBK114.96.
• Native Barcoding Kits: SQK-NBD114.24, SQK-NBD114.96.
• PCR Barcoding Kits: EXP-PBC001, EXP-PBC096, SQK-RPB114.24.
• cDNA Kits: SQK-PCS114, SQK-PCB114.24.
• Specialized Kits: 16S Barcoding Kit 24 V14, Ultra-Long Sequencing Kit V14.

Devices:

• MinION Mk1B
• MinION Mk1C
• GridION
• PromethION

Flow Cell Wash Kit:

• Enables reuse of the flow cell for multiple libraries.

Applications

1. High-Accuracy Sequencing:
   • Suitable for applications demanding high consensus accuracy, such as variant detection or clinical research.
2. Multiplexed Experiments:
   • Supports pooling of up to 96 samples using compatible barcoding kits.
3. Extended Workflows:
   • Ideal for incremental or large-scale sequencing projects requiring multiple libraries.
4. Reusable Design:
   • Enables efficient use of resources by supporting library washing and reloading.

Safety and Recycling

• Provided in a sealed pouch with a light shield.
• Returned flow cells are responsibly recycled.
• Safety Data Sheets available in multiple languages.

What’s in the Box

• Flow Cell (R10.4.1), sealed and packaged with:
  • Light shield for optimal sequencing conditions.
  • Returns kit for responsible recycling.

The SpotOn MinION Flow Cell (R10.4.1) is a reliable and high-accuracy tool for a variety of sequencing applications. Its compatibility with V14 kits and ONT devices makes it an essential component for modern sequencing workflows.