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Thermo Scientific™ Oxoid™ Membrane Lauryl Sulphate Broth (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Oxoid™ Membrane Lauryl Sulphate Broth (Dehydrated) is a selective medium designed for the enumeration of coliform organisms and Escherichia coli in water samples using the membrane filtration technique. This broth replaces Teepol with sodium lauryl sulphate, providing an effective and reproducible medium for water microbiology workflows.
Key Features
- Selective for Coliforms and E. coli:
- Sodium lauryl sulphate suppresses non-coliform organisms, ensuring selective growth of target organisms.
- Membrane Filtration Compatible:
- Ideal for quantitative testing of coliforms and Escherichia coli in water and sewage samples.
- Reliable and Reproducible:
- Consistent performance for enumeration of coliforms and E. coli.
- Validated Methodology:
- Recommended as a standard medium for water testing by multiple references.
- Dehydrated Format:
- Easy to prepare, with a yield of 6.6L per 500g package.
Applications
1. Water Microbiology
- Enumeration of coliforms and E. coli in potable water, wastewater, and recreational water.
2. Environmental Monitoring
- Detection of fecal contamination in environmental samples, including industrial effluents.
3. Public Health Compliance
- Monitoring water quality for regulatory standards to ensure safety and compliance.
Specifications
| Attribute | Details |
|---|---|
| Product Type | Dehydrated Culture Media |
| Form | Powder |
| Description | Membrane Lauryl Sulphate Broth |
| Applications | Water microbiology and environmental testing |
| Quantity | 500g |
| Yield | 6.6L of prepared medium |
Preparation Instructions
- Suspend: Suspend 76g of Membrane Lauryl Sulphate Broth powder in 1 liter of distilled water.
- Mix and Heat: Heat with frequent agitation until fully dissolved.
- Sterilize: Autoclave at 121°C for 15 minutes.
- Dispense: Pour into sterile containers for membrane filtration testing.
Advantages
- Highly Selective Medium: Suppresses non-target organisms, ensuring reliable recovery of coliforms and E. coli.
- Reproducible Results: Ensures consistent and accurate enumeration of target organisms.
- Broad Utility: Suitable for testing a variety of water sources, including potable and non-potable water.
- Ease of Use: Dehydrated format simplifies preparation for routine laboratory workflows.
Quality Control
| Control | Expected Results |
|---|---|
| Positive Control | Growth of coliform organisms and E. coli |
| Negative Control | Suppressed growth of non-target organisms |
Storage and Stability
- Storage: Store the dehydrated medium at 10–30°C in a dry environment.
- Shelf Life: Refer to the product label for expiration date.
Packaging
- Product: Membrane Lauryl Sulphate Broth (Dehydrated)
- Catalog Number: MM0615B
- Quantity: 500g
- Yield: 6.6L of prepared medium
The Thermo Scientific™ Oxoid™ Membrane Lauryl Sulphate Broth (Dehydrated) is a trusted and effective medium for the enumeration of coliforms and Escherichia coli in water samples. Its selective properties, reproducibility, and compatibility with membrane filtration make it an essential tool for laboratories focused on water quality testing, environmental monitoring, and public health compliance.