CellTiter-Glo® Luminescent Cell Viability Assay, MyGlo Reagent Reader

The CellTiter-Glo® Luminescent Cell Viability Assay, MyGlo Reagent Reader (MG1010) is an advanced system designed for seamless luminescence detection during ATP-based cell viability assays. The MyGlo Reagent Reader complements the CellTiter-Glo® Luminescent Cell Viability Assay, providing robust performance, accurate data collection, and user-friendly integration for high-throughput screening (HTS) and small-scale experiments.

Key Features of the MyGlo Reagent Reader:

1. Optimized for Luminescence Detection:
   • Designed specifically for luminescent assays, ensuring accurate ATP quantitation as a biomarker of cell viability.
2. Integrated Workflow:
   • Fully compatible with the CellTiter-Glo® Luminescent Cell Viability Assay, offering seamless detection and data capture.
3. High Sensitivity:
   • Detects as few as 15 cells per well in a 384-well format, with a dynamic linear range of up to 5 logs.
4. Automation-Compatible:
   • Integrates easily with robotic liquid handling systems for HTS environments, enabling batch processing and continuous workflows.
5. User-Friendly Interface:
   • Simplifies operation with intuitive software and quick assay setup, minimizing training requirements.
6. Compact and Portable:
   • Designed for use in small laboratory spaces without sacrificing performance or precision.

Applications:

1. Cell Viability Assays:
   • Quantifies the number of viable cells in culture based on ATP levels, a direct indicator of metabolic activity.
2. High-Throughput Screening (HTS):
   • Ideal for screening compounds in drug discovery and development programs.
3. Cytotoxicity Testing:
   • Measures the impact of test compounds, RNAi, or gene-editing tools on cell health and viability.
4. Cell Proliferation Studies:
   • Tracks growth rates and metabolic activity of cell populations under various experimental conditions.
5. Cancer Research:
   • Evaluates the efficacy of anticancer agents and gene knockdown experiments targeting tumor cells.
6. Antiviral Research:
   • Assesses cytopathic effects of viral infections, including SARS-CoV and SARS-CoV-2, in antiviral drug studies.

Advantages:

1. Enhanced Sensitivity:
   • Captures even low luminescent signals, enabling accurate detection of low cell numbers or minimal ATP levels.
2. Time Efficiency:
   • Facilitates rapid "add-mix-measure" workflows, with a signal half-life exceeding 5 hours for flexibility in processing.
3. Reproducible Results:
   • Ensures low variability (CV <10%) across wells for reliable data in both small and large-scale studies.
4. Streamlined Data Analysis:
   • Features intuitive software for rapid data capture, analysis, and export.
5. Cost-Effective:
   • Reduces reagent and sample consumption while maintaining high sensitivity, ideal for high-throughput settings.

Specifications:

System Components:

1. MyGlo Reagent Reader:
   • High-performance luminescence reader designed for compatibility with the CellTiter-Glo® Assay.
2. CellTiter-Glo® Reagent:
   • Detects ATP levels as a direct indicator of cell viability.
3. Intuitive Software:
   • Streamlines plate setup, data capture, and analysis for faster results.

Applications in Research and Industry:

1. Drug Discovery:
   • Screens large compound libraries for cytotoxic or therapeutic activity using high-throughput formats.
2. Gene Editing Validation:
   • Assesses the impact of CRISPR or RNAi-mediated knockdowns on cell viability and function.
3. Cancer Therapeutics:
   • Measures the efficacy of anticancer agents, including small molecules, biologics, and gene therapies.
4. Viral Pathogenesis:
   • Evaluates the cytopathic effects of viral infections and the efficacy of antiviral agents.

Use Cases:

1. Basic Research:
   • Tracks cellular metabolism and viability under various experimental conditions.
2. Biopharmaceutical Studies:
   • Monitors cell viability during biologic drug production processes.
3. Toxicology:
   • Evaluates the toxicity of environmental or industrial compounds on cultured cells.

Storage and Handling:

• Storage: Reagents should be stored at -20°C and protected from light.
• Maintenance: Ensure the MyGlo Reagent Reader is calibrated according to the manufacturer’s instructions for consistent performance.

Ordering Information:

The CellTiter-Glo® Luminescent Cell Viability Assay, MyGlo Reagent Reader (MG1010) is a comprehensive system for detecting ATP as a marker of cell viability. Its precision, ease of use, and compatibility with automated systems make it a valuable tool for high-throughput screening, cancer research, and drug development.