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Mabtech FluoroSpot Plus: Human IFN‑γ/Granzyme B/IL‑2
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Specifications:
| Application | ELISA-Based Assays, Fluorescence-Based Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Mabtech | ||
| Product Grade | Molecular Biology | ||
The FluoroSpot Plus: Human IFN-γ / Granzyme B / IL-2 Kit is a multiplex FluoroSpot assay designed to detect and quantify single cells secreting IFN-γ, Granzyme B, and IL-2 simultaneously. The assay utilizes fluorophore-tagged antibodies and high-affinity matched mAb pairs to ensure specific, sensitive, and reproducible detection of cytokine-secreting cells in human samples.
Ideal for immune profiling, T-cell functionality assays, vaccine response studies, and immuno-oncology research, this ready-to-use kit simplifies multiplex analysis with pre-coated plates and optimized reagents.
Key Features
- Detects three analytes (IFN-γ, Granzyme B, IL-2) from a single cell in one assay
- Uses high-specificity monoclonal antibodies for reliable cell-based cytokine detection
- Includes pre-coated FluoroSpot plates and matched detection antibodies
- Fluorophore-conjugated secondary antibodies for distinct, non-overlapping signal detection
- Supplied with FluoroSpot enhancer, positive controls (anti-CD3), and co-stimulatory reagents (anti-CD28)
- Optimized for human PBMCs, T cells, and effector cell populations
Kit Components
| Component | Details |
|---|---|
| Pre-coated Plate | FluoroSpot plate coated with anti-IFN-γ (1-D1K), anti-Granzyme B (MT34B6), and anti-IL-2 (MT2A91/2C95) antibodies |
| Detection Antibodies | Anti-IFN-γ mAb (7-B6-1, BAM), Anti-Granzyme B (MT28, biotin), Anti-IL-2 mAb (MT8G10, WASP) |
| Fluorophore Conjugates | Anti-BAM mAb-490, SA-550, Anti-WASP mAb-640 |
| Enhancer Solution | FluoroSpot Enhancer |
| Positive Control | Anti-CD3 mAb (CD3-2) |
| Co-stimulatory Reagent | Anti-CD28 mAb (CD28-A) |
Assay Details
| Assay Format | FluoroSpot (triplex) |
|---|---|
| Analytes Detected | Human IFN-γ, Granzyme B, IL-2 |
| Assay Application | Immune profiling, T-cell activation, vaccine and cancer immunotherapy research |
| Sensitivity | Single-cell cytokine detection |
| Sample Types | PBMCs, purified T cells, activated effector cells |
Shipping and Storage
| Shipping Condition | Ambient temperature |
|---|---|
| Storage Conditions |
- Antibodies, fluorophores, enhancer: 4–8 °C
- Plates: Room temperature
| Shelf Life | Minimum 12 months from date of receipt |
Ordering Information
| Catalog Number | Plate Quantity | Pack Size |
|---|---|---|
| FSP-013602-2 | 2 plates | 2 × pre-coated 96-well FluoroSpot plates |
| FSP-013602-10 | 10 plates | 10 × pre-coated 96-well FluoroSpot plates |
The FluoroSpot Plus: Human IFN-γ / Granzyme B / IL-2 Kit provides a robust platform for the simultaneous detection of three key immune effector molecules at the single-cell level. It supports detailed functional immune monitoring and is ideal for high-resolution multiplex analysis in immunological research.
- Pack Size: 2 Plates 10 Plates