Liofilchem™ Clostridium perfringens Agar Base

The Liofilchem™ Clostridium perfringens Agar Base is a highly specialized basal medium designed for the detection, enumeration, and differentiation of Clostridium perfringens from clinical specimens, food, water, and environmental samples. This medium conforms to ISO 7937, ISO 14189, and APHA standards, making it a reliable choice for microbiological testing across various industries.

Key Features

1. ISO-Compliant:
   • Meets the specifications of ISO 7937 and ISO 14189 for Clostridium perfringens detection, ensuring reliable results.
2. Versatile Usage:
   • Can be used with or without selective supplements such as:
     • D-Cycloserine (TSC Supplement).
     • Kanamycin/Polymyxin B Supplement.
     • D-Cycloserine 4-MUP Supplement for UV fluorescence detection.
3. Distinct Colony Appearance:
   • Produces black colonies due to the reaction between hydrogen sulfide (H₂S) and ferric ammonium citrate.
4. Nutrient-Rich Base:
   • Provides essential nutrients such as amino acids, nitrogen, and vitamins for optimal growth of clostridia.

Typical Formula (g/L)

Applications

1. Clinical Microbiology:
   • Detection and identification of Clostridium perfringens from stool, wound, and other clinical specimens.
2. Food and Water Testing:
   • Enumerates Clostridium perfringens in food products, animal feed, and drinking water.
3. Environmental Microbiology:
   • Monitors contamination in environmental samples to assess public health risks.

Preparation Instructions

1. Suspend 42g of powder in 1 liter of distilled or deionized water.
2. Heat to boiling, stirring until completely dissolved.
3. Autoclave at 121°C for 15 minutes.
4. Cool the medium to 45-50°C.
5. Add rehydrated selective supplements as needed:
   • T.S.C. Supplement (D-Cycloserine).
   • Kanamycin/Polymyxin B Supplement.
   • D-Cycloserine 4-MUP Supplement for fluorescence detection.
6. Mix thoroughly and pour into sterile Petri dishes.

Interpretation of Results

• Clostridium perfringens: Black colonies due to H₂S production.
• On TSC Agar with MUP: Light blue fluorescence under UV indicates Clostridium perfringens.
• Other Organisms:
  • Escherichia coli: Inhibited.
  • Bacillus subtilis: Inhibited.

Storage and Shelf Life

• Storage:
  • Store dehydrated medium at 10-30°C in a dry environment, tightly sealed.
  • Store prepared plates at 2-8°C, protected from light.
• Shelf Life:
  • Dehydrated medium: 4 years.
  • Prepared plates: Refer to specific product details.

Quality Control

Advantages

• Highly Selective: Inhibits unwanted flora while supporting Clostridium perfringens growth.
• Flexible: Can be used with various supplements to meet different testing needs.
• ISO-Compliant: Ensures reliability for food, water, and environmental testing.
• Distinct Differentiation: Black colonies and UV fluorescence allow clear identification.

Packaging

Warnings and Precautions

1. For professional use only.
2. Read all instructions carefully before use.
3. Avoid contamination by following standard laboratory practices.
4. Consult the Safety Data Sheet (SDS) for safe handling and disposal guidelines.

Why Choose Liofilchem Clostridium perfringens Agar Base?

• Trusted medium for reliable detection of Clostridium perfringens.
• Conforms to international standards for food, water, and environmental testing.
• Backed by Liofilchem’s expertise in microbiological media.