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Specifications:
| Application | Clinical microbiology |
| Storage Temperature | 2-8°C |
| Product Type | Microbial ID System |
| Product Brand | Liofilchem |
| Product Grade | Microbiology grade |
The Liofilchem™ Anaerobe System is a diagnostic tool designed for the biochemical identification of anaerobic microorganisms. It is particularly suited for clinical microbiology laboratories to accurately identify anaerobic pathogens responsible for various infections. This system provides reliable results within 24–48 hours, featuring cost-effective, easy-to-interpret testing with clear color changes.
Key Features
- Comprehensive Identification:
- Detects and identifies a wide range of clinically significant anaerobes, including:
- Actinomyces israelii
- Bacteroides fragilis
- Clostridium difficile
- Propionibacterium acnes
- Fusobacterium nucleatum and more.
- Detects and identifies a wide range of clinically significant anaerobes, including:
- Rapid and Reliable Results:
- Delivers accurate identification of anaerobic bacteria within 24–48 hours.
- Utilizes a panel of biochemical substrates for precise metabolic profiling.
- Cost-Effective and Efficient:
- Consolidates multiple biochemical tests into a single compact system.
- Reduces the need for additional reagents and consumables.
- Ease of Use:
- Features clear and well-defined color changes for straightforward interpretation.
- Compatible with manual or automatic reading systems.
Microorganisms Identified
| Genus | Species |
|---|---|
| Actinomyces | Actinomyces israelii |
| Bacteroides | B. fragilis, B. ovatus, B. thetaiotaomicron |
| Bifidobacterium | Bifidobacterium spp. |
| Clostridium | C. botulinum, C. sporogenes, C. difficile, C. perfringens |
| Eggerthella | Eggerthella lenta |
| Fusobacterium | F. necrophorum, F. nucleatum, F. varium |
| Lactobacillus | L. acidophilus, L. jensenii |
| Prevotella | P. melaninogenica, P. oralis |
| Propionibacterium | P. acnes |
| Staphylococcus | S. saccharolyticus |
| Streptococcus | S. intermedius |
Benefits
| Feature | Benefit |
|---|---|
| Complete Identification | Covers a wide range of clinically significant anaerobes. |
| Rapid Results | Provides results within 24–48 hours, supporting timely clinical decision-making. |
| Cost-Effective | Reduces laboratory costs by consolidating multiple tests into one system. |
| Clear Color Changes | Ensures easy interpretation, reducing the risk of errors. |
| Automation Compatible | Suitable for manual or automatic reading for increased laboratory efficiency. |
Test Procedure
- Inoculation:
- Prepare a bacterial suspension and inoculate the wells of the Anaerobe System panel.
- Incubation:
- Incubate the panel in anaerobic conditions at 36 ± 1°C for 24–48 hours.
- Result Interpretation:
- Observe color changes in the wells corresponding to biochemical reactions.
- Compare results with the reference guide to identify the microorganism.
- Identification:
- Generate a biochemical profile and use the provided codebook or software for species identification.
Applications
- Clinical Diagnostics:
- Identifies anaerobes associated with infections such as:
- Intra-abdominal abscesses.
- Dental infections.
- Clostridium-related diarrhea.
- Identifies anaerobes associated with infections such as:
- Industrial Microbiology:
- Monitors anaerobic contamination in pharmaceuticals and other industrial products.
- Research and Epidemiology:
- Studies the role of anaerobes in human microbiota and disease.
Packaging Information
| Product | Description | Reference |
|---|---|---|
| Anaerobe System | Biochemical ID panel for anaerobic bacteria | 71620 |
| Anaerobe System (Small) | Compact kit for lower-volume testing | 79620 |
Why Choose Liofilchem™ Anaerobe System?
- Comprehensive Testing: Identifies a broad spectrum of anaerobic microorganisms.
- Efficiency: Delivers fast, reliable results with minimal setup time.
- Ease of Use: Intuitive system with clear results interpretation.
- Flexibility: Suitable for clinical, research, and industrial microbiology applications.
Here’s a tabular comparison of the Liofilchem™ Anaerobe System and API 20A:
| Feature | Liofilchem™ Anaerobe System | API 20A |
|---|---|---|
| Manufacturer | Liofilchem S.r.l. | bioMérieux |
| Test Principle | Biochemical identification of anaerobic bacteria using a panel of biochemical substrates. | Miniaturized biochemical tests for identifying anaerobic bacteria. |
| Target Microorganisms | Anaerobic bacteria such as Clostridium difficile, Bacteroides fragilis, Propionibacterium acnes, and others. | Anaerobic bacteria, including Clostridium spp., Fusobacterium spp., and Peptostreptococcus spp. |
| Incubation Time | 24–48 hours. | 24–48 hours. |
| Result Interpretation | Color changes in wells indicate biochemical activity, supported by a codebook or automatic reading systems. | Color changes compared to a reference chart, with manual data entry for identification. |
| Automation Compatibility | Compatible with automatic reading systems for increased efficiency. | Primarily manual, though compatible with certain automation tools. |
| Ease of Use | User-friendly with clear and well-defined color changes, simplifying interpretation. | Requires careful comparison with reference colors; may involve more subjective analysis. |
| Scope of Identification | Broad range of anaerobes, including Bacteroides, Clostridium, Fusobacterium, and Prevotella. | Broad range of anaerobes, with strong emphasis on clinical pathogens. |
| Cost-Effectiveness | Noted for being a cost-effective solution for clinical laboratories. | Generally more expensive due to proprietary materials and established branding. |
| Accuracy | High accuracy with clear biochemical profiles; includes chromogenic technology for certain tests. | Good accuracy; studies report up to 71% species-level identification, with some limitations. |
| Additional Features | Offers automatic reading for high-throughput labs; supports manual interpretation with a codebook. | Relies on a robust database for species identification but lacks automatic reading integration. |
| Applications | Suitable for clinical diagnostics, industrial microbiology, and research labs. | Widely used in clinical diagnostics and research labs for anaerobic bacteria identification. |
Both the Liofilchem™ Anaerobe System and the API 20A are valuable tools for the identification of anaerobic bacteria. The choice between them may depend on specific laboratory needs, including desired accuracy, ease of use, cost considerations, and available equipment. Laboratories may also consider conducting in-house evaluations to determine which system aligns best with their diagnostic requirements.
- Pack Size: for 20 Tests for 4 Tests
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