Liofilchem® Tergitol 0.2% supplement

The Liofilchem TERGITOL 0.2% Supplement is a sterile liquid supplement used in microbiological applications to support the selective isolation and enumeration of coliform bacteria. When added to Tergitol + TTC Agar Base (Codes 610184 and 620184), the supplement enhances the medium's selectivity by inhibiting the growth of non-target organisms while promoting the growth of coliforms. It is widely utilized in water, food, and environmental testing to ensure accurate microbial enumeration.

Key Features

• Selective Enhancement: Contains Tergitol, a surfactant that inhibits Gram-positive organisms, thereby improving the recovery and differentiation of coliform bacteria.
• Ease of Use: Supplied as a ready-to-use sterile solution that integrates seamlessly with Tergitol + TTC Agar Base.
• Consistency: Prepared with stringent quality control to ensure reliable performance in microbial testing.
• Broad Applications: Designed for coliform isolation from water, food, and other environmental samples.

Composition

• Tergitol (0.2%): 2 g/L
• Water: 1000 mL

Applications

1. Water Testing:
   • Detect and enumerate coliforms in drinking water, recreational water, and wastewater.
   • Ensures compliance with public health regulations.
2. Food Microbiology:
   • Isolation of coliforms from processed foods, raw materials, and beverages.
   • Assists in ensuring food safety and hygiene.
3. Environmental Monitoring:
   • Used in laboratories to monitor microbial contamination in environmental samples.
4. Laboratory Research:
   • Widely used in microbiological studies to distinguish coliform bacteria.

Preparation & Usage Instructions

1. Preparation:
   • Aseptically take 5 mL of Tergitol 0.2% Supplement.
   • Add to 100 mL of Tergitol + TTC Agar Base (autoclaved and cooled to 50°C).
   • Mix thoroughly to ensure even distribution.
2. Dispensing:
   • Pour the prepared medium into sterile Petri dishes or other sterile containers.
3. Inoculation:
   • Apply the sample using the appropriate microbiological technique (e.g., membrane filtration or streaking).
   • Incubate plates according to the testing protocol.

Storage

• Store the product at 2-8°C in its original packaging.
• Avoid exposure to light and temperature fluctuations.
• Shelf Life: 2 years when stored under appropriate conditions.

Packaging

• 5 vials x 5 mL of Tergitol 0.2% Supplement.

Compatibility

• Specifically designed for use with Tergitol + TTC Agar Base (Codes 610184 and 620184).
• Compatible with coliform isolation workflows requiring selective media.

Quality Control

• Tested for sterility and performance.
• Validated for compatibility with Tergitol + TTC Agar Base.
• QC strains include Escherichia coli for performance verification.

References

1. Chapman, G.H. (1951). A culture medium for detecting and confirming Escherichia coli in ten hours. American Journal of Public Health, 41:1381.
2. Chapman, G.H. (1947). A superior culture medium for the enumeration and differentiation of coliforms. Journal of Bacteriology, 53:504.
3. Kulp, W., et al. (1953). Use of Tergitol-7 Triphenyl Tetrazolium Chloride Agar in routine sanitary water analysis. American Journal of Public Health, 43:1111.

Ordering Information

Safety Notes

• Intended for professional use only.
• Operators should have training in microbiological techniques.
• Follow all laboratory safety protocols.
• Consult the Safety Data Sheet (SDS) for detailed handling information.

This supplement is a critical component for selective microbial analysis, enabling accurate differentiation and enumeration of coliform bacteria in compliance with global microbiological standards.