Liofilchem® Penicillin G P, Antimicrobial Susceptibility Discs

The Liofilchem® Penicillin G P Antimicrobial Susceptibility Discs are precision-manufactured tools designed for the qualitative determination of bacterial susceptibility to Penicillin G. These discs are essential for clinical microbiology laboratories conducting routine antimicrobial susceptibility testing (AST) for a variety of gram-positive bacterial pathogens, including Streptococcus and Staphylococcus species.

Features and Benefits

1. Multiple Dosage Options:
   • Available in 1 IU, 2 IU, and 10 IU concentrations to meet diverse testing needs.
   • Flexibility in testing a wide range of bacterial strains with varying susceptibility.
2. Regulatory Compliance:
   • Conforms to international standards, including CLSI (M02) and EUCAST guidelines.
   • Manufactured under ISO 9001 and ISO 13485-certified quality systems.
3. High Accuracy and Reliability:
   • Ensures precise diffusion of Penicillin G for consistent results.
   • Performance validated against control strains for reproducibility.
4. Ease of Use:
   • Compatible with common AST media, such as Mueller-Hinton Agar.
   • Easy integration into routine laboratory workflows.
5. Cost-Effective Solution:
   • Each vial contains multiple discs, ensuring value for high-throughput laboratories.

Specifications

Testing Procedures

Preparation:

1. Prepare the test bacterial suspension to match the 0.5 McFarland standard (equivalent to 1.5 × 10⁸ CFU/mL).
2. Use a sterile swab to spread the suspension evenly onto the surface of a Mueller-Hinton Agar plate.

Disc Application:

1. Place the Penicillin G discs (1 IU, 2 IU, or 10 IU) onto the agar surface using sterile forceps.
2. Ensure even spacing between the discs to prevent overlapping inhibition zones.

Incubation:

1. Incubate the plates at 35 ± 2°C for 16–20 hours under aerobic conditions.

Interpretation:

1. Measure the diameter of the inhibition zones using a calibrated ruler.
2. Compare zone sizes with CLSI/EUCAST breakpoints to determine susceptibility.

Reference Data

Quality Control

The discs are tested with quality control strains to ensure consistent performance:

Regulatory and Validation Data

• CLSI Standards:
  • Conforms to CLSI M02 for disk diffusion susceptibility testing.
• EUCAST Compliance:
  • Aligns with EUCAST disk diffusion guidelines for Penicillin G susceptibility testing.
• ISO Certification:
  • Produced under ISO 20776-1 standards for antimicrobial susceptibility test systems.

Applications

1. Clinical Diagnostics:
   • Determines Penicillin G susceptibility for treating infections caused by Streptococcus and Staphylococcus species.
   • Assists in the management of respiratory infections, skin and soft tissue infections, and sepsis.
2. Pharmaceutical Research:
   • Evaluates Penicillin G efficacy in preclinical studies.
   • Tracks resistance trends among clinical isolates.
3. Antimicrobial Stewardship:
   • Supports efforts to optimize antibiotic usage and combat antimicrobial resistance.

Comparisons with Other Brands

The Liofilchem® Penicillin G P Antimicrobial Susceptibility Discs are an essential tool for reliable and efficient antimicrobial susceptibility testing. Their compliance with global standards, broad MIC range, and consistent quality make them ideal for clinical and research applications. These discs empower laboratories to deliver accurate results, aiding in the appropriate use of Penicillin G for treating bacterial infections.