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Specifications:
| Application | Clinical microbiology |
| Storage Temperature | 2-8°C |
| Product Type | Microbial ID System |
| Product Brand | Liofilchem |
| Product Grade | Microbiology grade |
The Liofilchem® Copro System Plus is an advanced diagnostic tool for detecting and presumptively identifying intestinal pathogenic microorganisms in fecal samples. The system features 18 wells containing desiccated biochemical substrates and provides a comprehensive method for identifying bacteria and fungi present in fecal specimens.
Key Features
- Comprehensive Identification: Detects Salmonella spp., Shigella spp., Proteus spp., Pseudomonas spp., Yersinia enterocolitica, Campylobacter jejunii, Escherichia coli, KES group microorganisms (Klebsiella spp., Enterobacter spp., Serratia spp.), and Candida spp..
- Multiple Tests: Includes lysine decarboxylation, hydrogen sulfide production, urea hydrolysis, indole, and Voges-Proskauer tests, alongside immunoserological agglutination tests.
- Rapid and Efficient: Provides results within 18-24 hours of incubation.
- Configurable Wells: Specific wells are designated for each pathogen, with reagents and color changes that facilitate interpretation.
Package Contents
- Ref. 71675: 20 systems + 20 physiological solution vials (7.0 mL each).
- Ref. 79675: 4 systems + 4 physiological solution vials (7.0 mL each).
- Test result forms and instructions.
Diagnostic Coverage
| Well | Test/Content | Microorganism | Positive Reaction |
|---|---|---|---|
| 1-LDC | Lysine decarboxylation | Salmonella spp. | Red |
| 2-ODC | Ornithine decarboxylation | Salmonella spp. | Red |
| 3-H2S | Hydrogen sulfide production | Salmonella spp. | Black |
| 4-SAL | Immunoserological test | Salmonella spp. | Agglutination |
| 5-PRO | Proteus/Providencia spp. growth | Proteus spp. | Brown-black |
| 6-UR | Urea hydrolysis | Proteus spp. | Red-fuchsia |
| 7-PSE | Pseudomonas spp. growth | Pseudomonas spp. | Turbid green |
| 8-OX | Cytochrome oxidase test | Pseudomonas spp. | Blue |
| 9-CIN | Yersinia enterocolitica growth | Yersinia enterocolitica | Turbid red |
| 10-YER | Immunoserological test | Yersinia enterocolitica | Agglutination |
| 11-SHI | Immunoserological test | Shigella spp. | Agglutination |
| 12-CAM | Immunoserological test | Campylobacter jejunii | Agglutination |
| 13-ESC | E. coli growth | Escherichia coli | Blue |
| 14-IND | Indole test | Escherichia coli | Pink-red ring |
| 15-KES | KES group growth | Klebsiella, Enterobacter, Serratia | Yellow |
| 16-VP | Voges-Proskauer test | KES group microorganisms | Pink-red |
| 17-CAN | Candida spp. growth | Candida spp. | Yellow |
| 18-OBS | Microscopic examination | Candida spp. | Presence of chlamydospores |
Test Procedure
- Preparation:
- Homogenize fecal specimens or prepare a rectal swab.
- Suspend in physiological solution, then transfer 0.5 mL to each well.
- Inoculation:
- Add vaseline oil to specific wells.
- Incubate at 36 ± 1°C for 18-24 hours.
- Interpretation:
- Observe color changes.
- Perform agglutination or biochemical confirmation tests where indicated.
Quality Control
Reference strains used to verify performance:
- Candida albicans (ATCC 10231)
- Proteus mirabilis (ATCC 25933)
- Escherichia coli (ATCC 25922)
- Salmonella typhimurium (ATCC 14028)
- Shigella flexneri (ATCC 12022)
- Pseudomonas aeruginosa (ATCC 27853)
Advantages
- Easy to use with preconfigured wells.
- Comprehensive microbial detection in a single test system.
- Suitable for both clinical and research laboratories.
Storage and Stability
- Store at 2–8°C in the original packaging.
- Avoid exposure to heat or rapid temperature changes.
- Shelf life: As indicated on the label.
Comparison with Similar Systems
| Feature | Liofilchem® Copro System Plus | BD BBL Enterotube II | Remel RapID System |
|---|---|---|---|
| Detection Scope | Bacteria & Fungi | Primarily Enterobacteriaceae | Biochemical profile-based bacteria |
| Time to Results | 18-24 hours | 24-48 hours | 4-6 hours |
| Number of Tests/Wells | 18 | 12 | 10 |
| Automation | Manual | Manual | Compatible with automated readers |
Here is a detailed comparison of the Liofilchem® Copro System Plus and the API 20E system, focusing on their features, applications, and performance.
Overview
| Feature | Liofilchem® Copro System Plus | API 20E |
|---|---|---|
| Purpose | Detection and presumptive identification of intestinal pathogenic microorganisms | Identification of Enterobacteriaceae and other Gram-negative rods |
| Target Pathogens | Broad spectrum: Salmonella spp., Shigella spp., Proteus spp., Pseudomonas spp., Yersinia enterocolitica, Campylobacter jejunii, Escherichia coli (incl. E. coli O157), KES group (Klebsiella, Enterobacter, Serratia), Candida spp. | Enterobacteriaceae and a limited range of other Gram-negative, oxidase-negative rods |
| System Type | Multitest biochemical and immunological system | Biochemical multitest system (20 tests) |
Key Features
| Feature | Liofilchem® Copro System Plus | API 20E |
|---|---|---|
| Number of Tests | 18 wells | 20 biochemical tests |
| Test Coverage | - Biochemical reactions: decarboxylation, hydrogen sulfide, indole, Voges-Proskauer - Immunoserological: latex agglutination for Salmonella, Shigella, Yersinia, Campylobacter, E. coli O157 - Microscopic confirmation: Candida spp. | Biochemical reactions only (e.g., decarboxylation, indole, citrate, urea hydrolysis, etc.) |
| Confirmation Steps | Includes serological agglutination and microscopic examination for fungi | Requires further confirmatory tests (e.g., oxidase test, additional media for specific pathogens) |
| Flexibility | Allows simultaneous testing for bacterial and fungal pathogens | Focused on Gram-negative bacteria only |
Performance
| Feature | Liofilchem® Copro System Plus | API 20E |
|---|---|---|
| Identification Accuracy | Presumptive identification with additional serological confirmation for pathogens like Salmonella, Shigella, and E. coli O157 | For Enterobacteriaceae and related organisms when used with identification databases |
| Incubation Time | 18–24 hours | 18–24 hours |
| Ease of Use | Requires additional reagents (e.g., latex kits, Vaseline oil, oxidase discs) | Requires immersion in saline suspension and use of analytical profile index (API) code for interpretation |
| Result Interpretation | Visual color change for presumptive identification, with serological and microscopic confirmation | Requires use of API reading strips or software for detailed identification |
Advantages
| Feature | Liofilchem® Copro System Plus | API 20E |
|---|---|---|
| Broad Detection Scope | Detects both pathogenic bacteria (Salmonella, Shigella, Yersinia) and fungi (Candida spp.) | Best suited for Enterobacteriaceae and oxidase-negative rods |
| Additional Pathogens | Includes tests for Campylobacter jejunii and Candida spp. | Lacks fungal and oxidase-positive pathogen detection |
| Confirmation Tests | Onboard wells for serological confirmation of key pathogens | Requires external media and reagents for additional testing |
| Cost-effectiveness | Ideal for laboratories handling diverse fecal pathogens | Highly effective for routine identification of Enterobacteriaceae |
Disadvantages
| Feature | Liofilchem® Copro System Plus | API 20E |
|---|---|---|
| Target Scope | Focuses primarily on intestinal pathogens; limited application outside gastrointestinal specimens | Limited to Gram-negative bacteria; excludes fungi and many oxidase-positive bacteria |
| Reagents Needed | Requires additional reagents for confirmation (e.g., Salmonella Latex Kit, Kovac’s reagent, Vaseline oil) | Requires saline preparation and database access for species-level identification |
| Time Investment | Manual setup for each pathogen and multiple interpretation steps | Simpler setup but dependent on external interpretation systems |
Applications
| Feature | Liofilchem® Copro System Plus | API 20E |
|---|---|---|
| Clinical Microbiology | Suitable for fecal cultures, including bacterial and fungal pathogens | Ideal for routine clinical identification of Gram-negative rods |
| Food and Water Testing | Detects foodborne pathogens like Salmonella and E. coli O157 | Applicable to Gram-negative contamination studies |
| Research | Useful for broader pathogen detection in gastrointestinal studies | Focused on Gram-negative bacterial diversity |
The Liofilchem® Copro System Plus offers a broader diagnostic scope, targeting both intestinal bacteria and fungi, and is ideal for laboratories handling fecal specimens with diverse pathogen profiles. The API 20E, on the other hand, is a specialized tool for detailed identification of Enterobacteriaceae and related Gram-negative rods, making it suitable for clinical and environmental microbiology labs focusing on bacterial pathogens.
Choose Liofilchem® Copro System Plus for:
- Broad-spectrum pathogen testing.
- Specific identification of gastrointestinal pathogens, including fungi.
Choose API 20E for:
- Detailed, high-accuracy identification of Enterobacteriaceae.
- Focused applications in clinical microbiology for Gram-negative bacteria.
The Liofilchem® Copro System Plus is a robust, multipurpose diagnostic tool for identifying pathogenic microorganisms from fecal samples. Its broad diagnostic scope, combined with ease of use, makes it ideal for clinical and research settings.
- Pack Size: for 20 Tests for 4 Tests