Liofilchem® CAMP Test, 30 Tests

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Specifications:

Application	Microbial Identification/AST
Storage Temperature	2-8°C
Product Type	Microbial ID System
Product Brand	Liofilchem
Product Grade	Microbiology grade

Overview
Resource

Rapid Presumptive Identification of Listeria monocytogenes and Group B Streptococci via Enhanced Hemolysis

The Liofilchem® CAMP Test is a ready-to-use diagnostic tool for detecting the CAMP factor—a hemolytic protein produced by Group B streptococci and some species of Listeria. The test enhances beta-hemolysis in the presence of Staphylococcus aureus or Rhodococcus equi, enabling rapid and visual confirmation of CAMP-positive organisms through a synergistic hemolytic reaction.

The discs are impregnated with viable, stabilized bacterial strains obtained from the American Type Culture Collection (ATCC) and are available in two formulations:

88021 – with Staphylococcus aureus ATCC 25923
88027 – with Rhodococcus equi ATCC 6939

The CAMP Test-R is widely used in clinical microbiology and food safety laboratories for the differentiation of Listeria monocytogenes from other Listeria species and for confirming Group B Streptococcus identification.

Key Features & Benefits

✅ Rapid and Easy-to-Use – Visual interpretation after 24-hour incubation
✅ Standardized Control Organisms – Discs impregnated with ATCC strains
✅ Reliable Differentiation – Detects enhanced hemolysis for identification of CAMP-positive strains
✅ Non-hazardous – Does not contain substances classified as dangerous under current legislation
✅ Versatile Application – Effective in both clinical and food microbiology testing
✅ Stability Verified – Maintains performance even after temporary storage at room temperature or short-term heat exposure
✅ In Vitro Diagnostic Use – Designed for professional microbiology laboratories

Product Specifications

Attribute	Details
Product Name	CAMP Test
Catalog Numbers	88021 (S. aureus ATCC 25923), 88027 (R. equi ATCC 6939)
Pack Size	30 Tests
Detection Target	CAMP factor (pyrrolidonyl-dependent hemolysis enhancer)
Test Method	Enhanced hemolysis on Tryptic Soy Agar + 5% Sheep Blood
Result Time	24 hours at 36 ± 1 °C
Readout	Purple zone of enhanced hemolysis = Positive test
Storage Conditions	2–8 °C (Stable for short-term transport at 18–25 °C for 4 days or 35–39 °C for 48 hours)
Shelf Life	See product label
Safety	Non-hazardous; refer to SDS for good laboratory practice
Disposal	Dispose in accordance with national/local regulations

Technique (Test Protocol)

Place a CAMP Test disc on a Tryptic Soy Agar + 5% Sheep Blood plate.
Moisten the disc with a drop of sterile saline.
Using a sterile loop, streak the test organism near the disc without touching it.
Incubate at 36 ± 1°C for 24 hours.
Observe for a hemolytic zone near the disc:
- Positive Test: Enhanced (synergistic) hemolysis near the disc
- Negative Test: No hemolytic enhancement

**Interpretation Table: CAMP Reaction for Listeria spp.**

Species	β-Hemolysis	CAMP Test
Listeria monocytogenes	+	+
Listeria innocua	–	–
Listeria ivanovii	+	–
Listeria seeligeri	+	+
Listeria welshimeri, L. grayi spp.	–	–
Jonesia denitrificans	–	–

Applications

🧫 Clinical Diagnostics – Identification of Group B Streptococci and Listeria monocytogenes

🥩 Food Safety Testing – Screening of dairy, meat, and ready-to-eat food for Listeria monocytogenes

🔬 Microbiology Research – Study CAMP factor and interspecies hemolytic interactions

📋 ISO-Compliant Testing – Aligned with ISO 11290-1:1996 for food microbiology

Ordering Information

Catalog #	Strain Impregnation	Pack Size
88021	Staphylococcus aureus ATCC 25923	30 Tests
88027	Rhodococcus equi ATCC 6939	30 Tests

The Liofilchem® CAMP Test provides a simple, accurate method for presumptive identification of Listeria monocytogenes and Group B Streptococci based on the CAMP phenomenon. With standardized ATCC strains, reliable hemolytic reaction, and ease of use, CAMP Test-R is a dependable choice for laboratories conducting routine microbial identification in clinical and food safety settings.