Liofilchem® Blood Agar (Sheep Blood 7%) 90mm Plates
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Specifications:
Application | Microbiology | ||
Storage Temperature | 2-8°C | ||
Product Type | Culture Medium | Forms | Solid |
Product Brand | Liofilchem | ||
Product Grade | Microbiology grade | ||
Ready-to-Use Culture Medium for Isolation, Cultivation, and Hemolysis Detection of Fastidious Organisms
Liofilchem® Blood Agar (Sheep Blood 7%) is a high-quality, ready-to-use microbiological medium formulated for the isolation and cultivation of fastidious pathogenic organisms and the detection of hemolytic activity. Enriched with 7% defibrinated sheep blood, this medium supports the growth of a broad range of microorganisms and is ideal for visualizing hemolytic reactions.
This formulation complies with ISO 7932 (confirmation of Bacillus cereus) and ISO 11290-1 (confirmation of Listeria monocytogenes), making it a trusted medium for food microbiology, clinical diagnostics, and reference laboratories.
Key Features & Benefits
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Enriched with 7% Sheep Blood
- Enhances growth of fastidious species and supports hemolysis detection
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Supports ISO Compliance
- Used for Bacillus cereus (ISO 7932) and Listeria monocytogenes (ISO 11290-1) confirmation
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Visible Hemolysis Differentiation
- Detects α-, β-, γ-, and ά-hemolysis for species identification
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High-Quality Ingredients
- Proteose peptone, liver digest, and yeast extract provide essential nutrients
- Sodium chloride balances osmotic pressure
- Agar solidifies the medium with an optimal cherry red, opaque appearance
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Ready-to-Use Format
- Available in 90 mm Petri dishes, pre-poured with 22 ± 1 mL of media
- Minimizes preparation time and contamination risk
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Excellent Shelf Life & Stability
- 75-day shelf life under recommended storage
- Stable during short-term temperature excursions in transport
Applications
- Isolation and identification of fastidious microorganisms
- Differentiation of hemolytic activity of Gram-positive cocci
- Clinical, veterinary, and food microbiology labs
- Diagnostic testing for Streptococcus, Staphylococcus, Listeria, Bacillus, and Clostridium species
Technical Specifications
Attribute | Specification |
---|---|
Medium Name | Blood Agar (Sheep Blood 7%) |
Format | Ready-to-use 90 mm Petri plates |
Volume per Plate | 22 ± 1 mL |
Appearance | Cherry red, opaque |
pH | 7.2 ± 0.2 at 25°C |
Shelf Life | 75 days from production date |
Storage | 2–8°C, protected from light |
Transport Stability | Stable for 4 days at 18–25°C or 48 hours at 35–39°C |
Sterilization | Gamma irradiated (filter-sterilized components) |
Microbiological Performance (QC Testing)
Organism (ATCC®) | Growth | Hemolysis Type |
---|---|---|
Staphylococcus aureus 25923 | Good | β-hemolysis |
Streptococcus pyogenes 19615* | Good | β-hemolysis |
Streptococcus pneumoniae 6303* | Good | α-hemolysis |
Neisseria meningitidis 13090* | Good | γ-hemolysis |
Listeria monocytogenes 19118 | Good | β-hemolysis |
Bacillus cereus 11778 | Good | β-hemolysis |
Escherichia coli 25922 | Good | None |
Clostridium perfringens 13124** | Good | β-hemolysis |
*Requires 10% CO₂ incubation
**Anaerobic incubation required
Ordering Information
Catalog No. | Content | Packaging |
---|---|---|
10142 | 20 plates | 2 × 10 plates sealed in thermal film, boxed |
10142* | 100 plates | 10 × 10 plates in sealed film, boxed |
Liofilchem® Blood Agar (Sheep Blood 7%) is a reliable, ISO-compliant culture medium for the growth and hemolytic differentiation of fastidious and pathogenic microorganisms. Trusted in clinical and food microbiology, this ready-to-use format enhances accuracy, reproducibility, and efficiency in bacterial identification workflows.
- Pack Size: 20/Box 100/Box