Invitrogen™ PureLink™ Pro 96 Genomic DNA Purification Kit, 4 x 96 Preps
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Specifications:
Application | DNA Extraction and Purification | ||
Storage Temperature | Room Temperature, 2-8°C | ||
Product Type | Sample Prep | Forms | Kit with Various components |
Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Pro 96 Genomic DNA Purification Kit provides a high-throughput solution for isolating high-yield, high-purity genomic DNA (gDNA) from a wide range of sample types. Designed for use in a 96-well silica plate format, this kit enables parallel processing of up to 96 samples in ~45 minutes, ensuring efficiency and reproducibility for large-scale projects.
With its optimized buffers and advanced plate design, the kit delivers A260/A280 and A260/A230 absorbance ratios of ~1.8, indicating DNA free from protein, RNA, guanidine, or other contaminants. The semi-skirted plate with annular collar nozzles prevents cross-contamination and improves drying, minimizing ethanol carryover—crucial for downstream success.
Key Features
- Flexible Sample Compatibility – One kit supports gDNA isolation from blood, tissue, cells, FFPE, saliva, buccal swabs, blood spots, bacterial and yeast cells, and tail biopsies.
- High Quality Output – Consistently high-purity gDNA with clean absorbance ratios.
- High Throughput – 96-well plate format enables manual or automated processing.
- Improved Design – Semi-skirted plates compatible with centrifugation, benchtop vacuums, and robotic liquid handling systems.
- Fast Workflow – DNA ready for use in ~45 minutes, including homogenization.
Applications
- PCR amplification and qPCR.
- DNA sequencing and NGS library preparation.
- Southern blotting and nucleic acid hybridization.
- Cloning and molecular biology research.
- Long-term genomic studies using high-purity gDNA.
Technical Specifications
Parameter | Details |
---|---|
Product Type | Genomic DNA Purification Kit (96-well) |
Catalog Number | K182104A (4 × 96 preps) |
Isolation Technology | Silica plate |
Sample Types | Blood, tissues, cells, FFPE, saliva, buccal swabs, blood spots, bacteria, yeast, mouse/rat tails |
Sample Input | ≤25 mg tissue, ≤200 µL blood, ≤10⁹ bacterial cells, ≤10⁷ yeast cells |
Yield | Up to 0.5 mg gDNA (binding capacity) |
DNA Quality | A260/A280 and A260/A230 ~1.8 |
Elution Volume | 50–200 µL |
Test Time | ~45 minutes (including homogenization) |
Format | 96-well plate |
Prep Scale | Mini-prep scale per well |
High-Throughput | Compatible with centrifuge, vacuum manifold, and robotic automation |
Shipping Condition | Room temperature |
Storage | Buffers at RT; Proteinase K at 4 °C for long-term storage |
Unit Size | Each kit (4 × 96 preps) |
Kit Contents
- PureLink Pro 96 Genomic Lysis/Binding Buffer
- Digestion Buffer
- Wash Buffers 1 & 2
- Elution Buffer
- RNase A
- Proteinase K
- Genomic DNA Filter Plates
- 96 Deep Well Blocks
- Foil Tape
Unique Advantages
- One kit for multiple sample sources, reducing the need for specialized kits.
- Optimized buffer system ensures reproducibility across diverse inputs.
- Suitable for both manual labs and automated robotic workflows.
- Outperforms competitor kits in both DNA yield and purity.
The Invitrogen™ PureLink™ Pro 96 Genomic DNA Purification Kit is a robust, high-throughput solution for extracting high-quality gDNA from diverse biological sources. Delivering fast, consistent, and ultraclean DNA, this kit is ideal for laboratories conducting large-scale genomic studies, sequencing projects, or molecular biology workflows requiring reliable gDNA.