Invitrogen™ Lipofectin™ Transfection Reagent

Invitrogen™ Lipofectin™ Transfection Reagent is a lipid-based transfection reagent specifically formulated for efficient delivery of DNA, RNA, and oligonucleotides into mammalian cells, including endothelial cells, primary cells, and hard-to-transfect cell lines. It is also suitable for DNA transfection into plant protoplasts and works well in combination with PLUS™ Reagent for enhanced transfection efficiency in HeLa cells.

Key Features:

1. Broad Applicability:
   • Suitable for transfecting DNA, RNA, and oligonucleotides into a wide variety of cell types.
   • Effective for DNA transfection into plant protoplasts.
2. Optimized Liposome Formulation:
   • A 1:1 (w/w) blend of N-[1-(2,3-dioleyloxy)propyl]-n,n,n-trimethylammonium chloride (DOTMA) and dioleoyl phosphatidylethanolamine (DOPE).
3. Efficient for Hard-to-Transfect Cells:
   • Proven efficiency in endothelial cells and primary cells.
4. Compatible with PLUS™ Reagent:
   • Enhances transfection performance in HeLa cells and other established cell lines.
5. Ready-to-Use:
   • Supplied in a liquid form for ease of use in multiple plate formats and flasks.

Specifications:

Applications:

1. Transfection of Endothelial Cells:
   • Optimized for delivering nucleic acids into endothelial cells for gene expression and functional studies.
2. Gene Delivery:
   • Facilitates the transfection of DNA, RNA, and oligonucleotides into a wide range of mammalian cells.
3. Plant Research:
   • Suitable for introducing DNA into plant protoplasts, enabling plant genetic studies.
4. Hard-to-Transfect Cells:
   • Efficiently delivers nucleic acids into primary and challenging cell types.
5. High-Efficiency Transfection:
   • Works synergistically with PLUS™ Reagent for enhanced transfection in HeLa cells and similar cell lines.

Protocol Overview:

1. Prepare Transfection Complex:
   • Dilute DNA and Lipofectin™ Reagent in serum-free medium (e.g., Opti-MEM).
2. Incubation:
   • Incubate the mixture at room temperature for 20–30 minutes to allow complex formation.
3. Apply to Cells:
   • Add the transfection complex to cells in serum-free medium.
4. Incubate and Replace Medium:
   • Incubate for 4–6 hours at 37°C, then replace the medium with complete growth medium.
5. Monitor Results:
   • Assess transfection efficiency via downstream assays (e.g., fluorescence or gene expression analysis).

Ordering Information:

Storage and Handling:

• Storage: Store at 4°C. Do not freeze.
• Shelf Life: Refer to the product label for expiration details.
• Shipping: Ships on wet ice.

Advantages:

1. High Transfection Efficiency:
   • Optimized lipid formulation ensures reliable delivery of nucleic acids.
2. Versatile Usage:
   • Works across various cell types and nucleic acid types.
3. Simplified Protocol:
   • Easy-to-follow procedure compatible with multiple formats.
4. Synergy with PLUS™ Reagent:
   • Enhanced performance when combined with PLUS™ Reagent for challenging applications.
5. Non-Toxic:
   • Gentle on cells, reducing cytotoxicity during transfection.

Related Products:

• Opti-MEM Reduced Serum Medium: Ideal for use with Lipofectin™ during transfection.
• Invitrogen™ PLUS™ Reagent: Enhances transfection efficiency in difficult cell lines.
• Gibco™ DMEM: For culturing cells pre- and post-transfection.

Regulatory Notes:

• For Research Use Only: Not for use in diagnostic or therapeutic procedures.

The Invitrogen™ Lipofectin™ Transfection Reagent provides a robust, flexible solution for transfecting various nucleic acids into mammalian and plant cells. Its compatibility with a wide range of cell types and synergy with PLUS™ Reagent make it an essential tool for gene expression and molecular biology studies.