Gibco™ DMEM, powder, high glucose

Gibco™ Dulbecco's Modified Eagle Medium (DMEM) is a high-quality basal medium widely used in mammalian cell culture. It is specifically formulated to support the growth of various cell types, including primary cells like fibroblasts and neurons, as well as established cell lines such as HeLa, 293, Cos-7, and PC-12. DMEM offers an optimal balance of amino acids, vitamins, glucose, and buffering systems for efficient cell growth and maintenance.

Key Features:

1. High-Glucose Content:
   • Designed for energy-demanding cell types.
   • Enhanced glucose concentration supports rapid growth and proliferation.
2. Additives:
   • Included: L-glutamine, phenol red (pH indicator), high glucose.
   • Excluded: Sodium pyruvate, HEPES buffer, sodium bicarbonate.
3. Flexible Usage:
   • Can be customized with user-preferred supplements (e.g., sodium bicarbonate, FBS).
   • Requires a 5-10% CO₂ environment for maintaining physiological pH.
4. Customizable Formulation:
   • The powdered format allows for flexible preparation, pH adjustment, and sterilization as per specific research needs.
5. Quality Assurance:
   • Manufactured in a cGMP-compliant facility.
   • Meets ISO 13485 standards ensuring consistency and safety for cell culture applications.

Applications:

1. Mammalian Cell Culture:
   • Suitable for culturing primary cells (e.g., fibroblasts, neurons, glial cells) and established cell lines (e.g., HeLa, Cos-7).
2. Research Areas:
   • Cellular metabolism studies.
   • Transfection and gene expression experiments.
   • Cytotoxicity assays.
3. Tissue Engineering and Biopharma:
   • Used in early-phase biopharmaceutical development.

Specifications:

Preparation Protocol (Powder Form):

1. Dissolve the required amount of DMEM powder in distilled water.
2. Add sodium bicarbonate as per the formulation requirements.
3. Adjust the pH to 7.2-7.4 using a pH meter.
4. Sterilize the prepared medium using filtration (0.22 μm filter recommended).
5. Store at 2-8°C and use within the recommended shelf life.

Catalog Numbers and Sizes:

• 12100061: 10 L
• 52100021 (52100-021): 5 L
• 52100039 (52100-039): 10 L
• 52100047 (52100-047): 50 L

Key Benefits:

• Versatility in supporting a wide range of cell types.
• Customizable powder formulation for flexibility in experimental setups.
• High-glucose content for enhanced cell performance in energy-demanding applications.

For researchers in cell biology, biopharma, and translational medicine, Gibco™ DMEM, Powder, High Glucose is a trusted solution for achieving reliable and reproducible results in cell culture.