Invitrogen™ Collibri™ PCR-free PS DNA Library Prep Kit for Illumina Systems

The Invitrogen™ Collibri™ PCR-free PS DNA Library Prep Kit for Illumina™ Systems is a premium next-generation sequencing (NGS) library preparation solution designed to generate highly accurate DNA libraries without PCR amplification. By eliminating PCR amplification bias and errors, this kit enables superior sequencing fidelity, uniform genome coverage, and improved detection of SNPs, INDELs, and structural variants.

Optimized for Illumina sequencing platforms, this PCR-free workflow is ideal for whole genome sequencing, resequencing, and de novo assembly applications where maximum sequence accuracy and minimal library bias are essential. The kit supports physically sheared genomic DNA, cell-free DNA (cfDNA), and amplicons across a wide range of species, delivering high-quality libraries suitable for demanding genomic studies.

Key Features and Benefits

PCR-Free Workflow for Maximum Accuracy

• Eliminates PCR-induced bias and amplification errors
• Improves detection of SNPs, INDELs, and structural variants
• Provides highly uniform genome coverage

Superior Sequencing Performance

• Ideal for high-resolution whole genome sequencing
• Improves coverage of GC-rich and difficult genomic regions
• Generates highly reproducible sequencing libraries

High DNA Input Compatibility

• Supports input DNA ≥500 ng
• Compatible with:
  • Fragmented genomic DNA
  • Cell-free DNA (cfDNA)
  • Amplicons ≤300 bp
  • DNA from any species

Fast and Efficient Workflow

• Library preparation completed in approximately 3 hours
• Reduced hands-on time and simplified workflow

Automation-Compatible Design

• Suitable for high-throughput sequencing workflows
• Compatible with automated liquid handling systems

Visual Workflow Tracking

• Built-in inert dyes provide visual cues during library preparation
• Improves workflow accuracy and reduces pipetting errors

Flexible Multiplexing Options

• Available with combinatorial dual indexes (CD Indexes)
• Available with unique dual indexes (UD Indexes)
• Supports multiplex sequencing of up to 96 samples

Applications

The Collibri PCR-free PS DNA Library Prep Kit is ideal for:

• Whole genome sequencing (WGS)
• Human genome sequencing
• Cancer genomics
• Population genomics
• Rare variant detection
• Structural variant analysis
• De novo genome assembly
• Clinical genomics research
• High-accuracy resequencing
• Cell-free DNA sequencing

Illumina Sequencing Platform Compatibility

Fully compatible with Illumina sequencing systems including:

• NovaSeq™ 6000
• NextSeq™ 550
• NextSeq™ 1000 and 2000
• HiSeq™ X, 2500, 3000, and 4000
• MiSeq™
• iSeq™

Available Kit Variants and Configurations

Index Types:

• CD Indexes: Combinatorial Dual Indexes
• UD Indexes: Unique Dual Indexes (recommended for highest multiplexing accuracy)

Workflow Overview

Typical PCR-free workflow steps include:

1. DNA fragmentation (if required)
2. End repair and adapter ligation
3. Cleanup and size selection
4. Index incorporation
5. Generation of sequencing-ready libraries

Technical Specifications

Advantages Over PCR-Based Library Preparation Kits

• Eliminates PCR bias and amplification artifacts
• Improves variant calling accuracy
• Provides superior genome coverage uniformity
• Reduces sequencing errors
• Improves reproducibility
• Ideal for high-precision genomic studies

The Invitrogen™ Collibri™ PCR-free PS DNA Library Prep Kit for Illumina™ Systems provides an advanced, high-fidelity solution for DNA library preparation in next-generation sequencing workflows. By eliminating PCR amplification bias, this kit delivers unmatched accuracy, uniform coverage, and reliable variant detection, making it an ideal choice for whole genome sequencing, clinical genomics research, and precision genomics applications. Its automation compatibility, flexible indexing options, and optimized workflow ensure efficient and reproducible sequencing library preparation across diverse genomic research environments.