Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Core Kit for Illumina™ Systems

The Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Core Kit for Illumina Systems is a premium next-generation sequencing (NGS) library preparation solution designed to generate high-quality DNA libraries without PCR amplification. By eliminating PCR, this kit minimizes amplification bias, reduces duplicate reads, and preserves the original representation of the genome, making it ideal for high-accuracy sequencing applications such as whole genome sequencing and variant detection.

The kit utilizes advanced enzymatic fragmentation (ES) technology to produce consistent DNA fragment sizes and high library yields from input DNA amounts starting at 100 ng. The streamlined PCR-free workflow can be completed in approximately two hours, significantly improving laboratory efficiency while maintaining excellent sequencing performance.

As a Core Kit, it provides enzymatic fragmentation and cleanup modules, allowing users to combine with their preferred Collibri indexed adapters for customized multiplex sequencing workflows on Illumina platforms including NovaSeq™, NextSeq™, MiSeq™, and HiSeq™ systems.

Key Features

PCR-free Workflow for Maximum Sequencing Accuracy

• Eliminates PCR amplification bias and sequence distortion
• Reduces duplicate reads and improves data quality
• Preserves true genomic representation

Fast and Efficient Workflow

• Complete library preparation in approximately 2 hours
• Reduced hands-on time improves laboratory productivity
• Streamlined protocol simplifies sequencing workflows

Advanced Enzymatic Fragmentation Technology

• Controlled enzymatic shearing ensures consistent DNA fragmentation
• Generates high-yield libraries suitable for sequencing and quantification
• Provides reliable and reproducible performance

Wide Sample Compatibility

• Supports genomic DNA inputs ≥100 ng
• Compatible with amplicons larger than 300 bp
• Suitable for DNA samples from all species

Flexible Core Kit Configuration

• Allows use with preferred Collibri indexing adapters
• Supports customized multiplex sequencing workflows

Automation and High-Throughput Ready

• Compatible with automated liquid handling systems
• Supports miniaturized workflows to reduce reagent costs
• Ideal for high-throughput sequencing laboratories

Visual Workflow Monitoring

• Inert dyes provide visual confirmation of workflow progress
• Improves accuracy and reduces preparation errors

Applications

The Collibri PCR-free ES DNA Library Prep Core Kit is ideal for demanding sequencing applications requiring high accuracy and minimal bias, including:

• Whole genome sequencing (WGS)
• Genome resequencing
• De novo genome assembly
• Variant detection and SNP analysis
• Population genomics
• Cancer genomics research
• Microbial genomics
• Precision medicine research

Illumina Platform Compatibility

Fully compatible with Illumina next-generation sequencing systems, including:

• NovaSeq™ 6000
• NextSeq™ 550
• MiSeq™
• iSeq™
• HiSeq™ X, 2500, 3000, and 4000

Kit Contents and Storage

Ships on dry ice to preserve reagent stability.

Technical Specifications

Advantages for Sequencing Laboratories

• Eliminates PCR bias for improved sequencing accuracy
• Preserves genomic complexity and variant representation
• Faster workflow compared to traditional PCR-based methods
• High library yield and reproducibility
• Compatible with Illumina NovaSeq, NextSeq, MiSeq, and HiSeq platforms
• Ideal for high-precision genomics and research applications

The Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Core Kit for Illumina Systems provides a fast, reliable, and highly accurate solution for preparing sequencing libraries without PCR amplification. Its enzymatic fragmentation technology, streamlined workflow, and automation compatibility make it an ideal choice for genomics laboratories, sequencing core facilities, and research institutions requiring high-fidelity DNA sequencing and unbiased genomic analysis.