Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

The Invitrogen™ UltraPure™ Phenol:Chloroform Alcohol (25:24:1) is a molecular biology-grade reagent widely used in nucleic acid purification. This solution ensures efficient separation of nucleic acids from proteins and other contaminants, yielding high-quality DNA and RNA suitable for downstream applications.

Key Features

1. High Purity:
   • Molecular biology-grade reagent, ensuring minimal contamination for sensitive nucleic acid workflows.
2. Efficient Phase Separation:
   • Denatures proteins and separates them into the organic phase or interphase while retaining nucleic acids in the aqueous phase.
3. Ready-to-Use:
   • Pre-saturated with Tris-HCl at pH 8.05 for optimal performance in nucleic acid purification.
4. Preservative-Free:
   • Contains no preservatives, reducing the risk of unwanted chemical interference.
5. Enhanced Stability:
   • Packaged under inert gas in amber bottles to maintain stability and protect from light.

Specifications

Applications

1. DNA Extraction:
   • Purify genomic DNA from complex biological samples.
2. RNA Extraction:
   • Isolate high-quality RNA from cells and tissues for transcriptomic studies.
3. Protein Removal:
   • Denature and separate proteins during nucleic acid isolation workflows.
4. Sample Preparation:
   • Prepare high-purity nucleic acids for PCR, qPCR, sequencing, or cloning.

Advantages

Workflow

1. Sample Preparation:
   • Add equal volumes of the reagent and the nucleic acid sample.
2. Mix and Centrifuge:
   • Vortex the mixture thoroughly and centrifuge to separate phases.
3. Phase Recovery:
   • Collect the upper aqueous phase containing nucleic acids.
4. Repeat (if necessary):
   • Perform additional extractions for improved purity.
5. Storage:
   • Store purified nucleic acids at -20°C or -80°C for long-term use.

Comparison with Competitor Products

Suggested Pairing

• RNA Extraction Kits:
  • Combine with Invitrogen™ TRIzol™ Reagent for RNA isolation workflows.
• DNA Extraction Kits:
  • Use with Invitrogen™ PureLink™ Genomic DNA Kits for high-yield DNA purification.
• Downstream Applications:
  • Pair with Invitrogen™ Platinum™ Taq DNA Polymerase for reliable PCR amplification.

The Invitrogen™ UltraPure™ Phenol:Chloroform Alcohol (25:24:1) is a trusted solution for high-quality nucleic acid extraction, offering excellent performance and reliability for molecular biology research applications.