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Invitrogen™ UltraComp eBeads™ Plus Compensation Beads
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | beads | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ UltraComp eBeads™ Plus Compensation Beads are next-generation compensation beads engineered to support accurate, reproducible compensation controls in multicolor flow cytometry. These beads are designed to mimic the optical properties of cells more closely than first-generation beads, especially when used with Brilliant Violet™ and Super Bright™ fluorochromes.
Each bead suspension contains two distinct populations:
- Positive beads that bind to fluorochrome-conjugated antibodies
- Negative beads that do not bind antibodies
This bimodal fluorescence distribution facilitates precise single-color compensation setup and minimizes spectral overlap, allowing users to confidently configure detectors for complex multicolor panels.
Key Features and Benefits
- Multispecies Compatibility: Binds antibodies from mouse, rat, hamster, rabbit, and recombinant human sources
- Broad Fluorochrome Compatibility: Suitable for fluorophores excited by UV (355 nm), violet (405 nm), blue (488 nm), green (532 nm), yellow-green (561 nm), and red (633–640 nm) lasers
- Enhanced Performance: Better mimics cell-based compensation with Brilliant Violet™ 785/786, Brilliant Violet™ 711, Super Bright™ 780, and Super Bright™ 702 conjugates
- Uniform Bead Size: 5 µm beads ensure consistent scatter properties across tests
- Bimodal Fluorescence Output: Clearly distinguishes between positive and negative populations for accurate compensation gating
- Convenient Format: Ready-to-use formulation; just add fluorochrome-conjugated antibodies
Technical Specifications
| Property | Specification |
|---|---|
| Product Type | Compensation Beads |
| Bead Size | 5 µm (spherical particles) |
| Concentration | 5 × 10⁶ beads/mL |
| Excitation/Emission | UV to Red lasers (355–640 nm) / ~635 nm |
| Antibody Compatibility | Mouse, rat, hamster, rabbit, recombinant human |
| Fluorophore Compatibility | Brilliant Violet™, Super Bright™, and standard dyes |
| No. of Tests | 25 or 100 (depending on catalog number) |
| Format | Liquid, ready-to-use |
| Storage Conditions | Store per manufacturer’s recommendations |
| Unit Size | Each |
Applications
- Single-color compensation setup in flow cytometry
- Multicolor panel validation
- Fluorochrome spectral overlap correction
- Reagent optimization and instrument calibration
- Compensation control for rare cell populations
UltraComp eBeads™ Plus Compensation Beads from Invitrogen are an essential reagent for researchers using multicolor flow cytometry. With expanded antibody compatibility, broad fluorochrome applicability, and a cell-like compensation profile, these beads enable precise, reproducible instrument setup for even the most complex fluorescence panels. They are particularly valuable for panels incorporating Brilliant Violet™ or Super Bright™ dyes, where standard compensation beads may fall short.
- Pack Size: For 100 Tests for 25 Tests