Invitrogen™ Tris (1 M), pH 8.0, RNase-free

The Invitrogen™ Ambion™ 1 M Tris Buffer (pH 8.0, RNase-Free) is a high-purity, pre-mixed buffer designed for molecular biology applications. This ready-to-use Tris buffer is certified RNase-free, ensuring a contamination-free environment for RNA and DNA research. It is widely used in nucleic acid purification, PCR, qPCR, RT-PCR, and electrophoresis buffer systems.

Tris buffer is a critical component in biological and biochemical experiments, providing pH stability and buffering capacity over a range of 7.0–9.0. The pH 8.0 formulation makes it ideal for RNA and DNA-based applications, preventing acidic degradation of nucleic acids.

Key Features & Benefits

1. High-Purity, RNase-Free Molecular Biology Grade

• Certified free of RNase, DNase, and proteases, making it ideal for RNA research.
• Manufactured under nuclease-free conditions to prevent contamination.
• Rigorously tested for non-specific endonuclease and exonuclease activity.

2. Ready-to-Use 1 M Tris Buffer (pH 8.0)

• Pre-mixed and pH-adjusted, eliminating preparation time and errors.
• Provides stable buffering capacity to maintain optimal pH conditions.
• Economical and convenient for high-throughput molecular biology applications.

3. Essential Reagent for Nucleic Acid and Protein Research

• Maintains RNA and DNA integrity in nucleic acid extractions.
• Used in electrophoresis buffers, PCR, and hybridization reactions.
• Commonly found in TE buffer (Tris-EDTA) for DNA storage and sequencing.

Specifications

Applications

1. Molecular Biology & RNA Applications

• Maintains RNA integrity in RNA extraction and storage.
• Used in hybridization buffers for Northern and Southern blotting.
• Provides a stable pH environment for enzymatic reactions.

2. PCR, qPCR, and RT-PCR Buffer Systems

• Key component in PCR and RT-PCR buffers to stabilize polymerase reactions.
• Used in cDNA synthesis and sequencing workflows.
• Ensures consistent pH for optimal primer annealing and extension.

3. Electrophoresis & Nucleic Acid Gel Buffers

• Used in TAE (Tris-Acetate-EDTA) and TBE (Tris-Borate-EDTA) buffers.
• Ensures consistent pH conditions during agarose and PAGE gel electrophoresis.
• Prevents DNA degradation in long electrophoresis runs.

4. Protein Biochemistry & Enzyme Reactions

• Used in protein purification and Western blot buffers.
• Maintains pH stability in enzyme reactions involving DNA ligases, polymerases, and restriction enzymes.

Pack Sizes & Catalog Numbers

Storage & Handling

• Store at room temperature for long-term stability.
• Use sterile techniques when handling to prevent contamination.
• Keep tightly sealed to maintain RNase-free conditions.

Summary

The Invitrogen™ Ambion™ 1 M Tris Buffer, pH 8.0 is a high-quality, pre-mixed, RNase-free buffer optimized for RNA and DNA research, PCR, electrophoresis, and enzyme reactions. Its ready-to-use format ensures accurate pH control and contamination-free use, making it an essential reagent for molecular biology applications.