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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | -20°C | ||
| Product Type | Sample Prep | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ Ni-NTA Agarose is a nickel-charged affinity resin specifically designed for purifying recombinant proteins containing a polyhistidine tag (6xHis). The resin employs nitrilotriacetic acid (NTA) as the chelating ligand, coupled to a cross-linked 6% agarose matrix. This versatile product supports purification under both native and denaturing conditions and is suitable for various applications, including batch and gravity flow methods.
Key Features
- High Binding Capacity:
- Binds up to 50 mg of recombinant protein per 1 mL of resin, making it highly efficient for purification workflows.
- Versatile Applications:
- Compatible with both native and denaturing conditions, allowing flexibility for different experimental requirements.
- Robust and Stable:
- Provided as a 50% slurry in 30% ethanol, ensuring easy handling.
- Resin stability guaranteed for 6 months when stored properly at +4°C.
- Easy Elution:
- Proteins can be eluted using low pH buffers, or by competition with imidazole or histidine, ensuring high-purity recovery.
- Distinctive Appearance:
- Resin is blue in color when charged with Ni²⁺, providing a visual confirmation of its active state.
Specifications
| Specification | Details |
|---|---|
| Stationary Phase | Ni-NTA |
| Matrix Type | 6% Cross-linked Agarose |
| Form | Suspension |
| Column Type | Affinity Column |
| Binding Capacity | Up to 50 mg protein per 1 mL of resin |
| Slurry Composition | 50% slurry in 30% ethanol |
| Stability | 6 months at +4°C |
| Storage Temperature | +4°C |
| Unit Sizes | 10 mL, 25 mL, 100 mL |
| Applications | Batch and Gravity Flow Purification |
Applications
- Purification of His-Tagged Proteins:
- Ideal for recombinant protein purification containing 6xHis tags.
- Flexible Binding Conditions:
- Enables purification under native conditions for functional proteins or denaturing conditions for insoluble proteins.
- Research and Development:
- Suitable for structural biology, protein-protein interaction studies, and functional assays.
- High-Yield Protein Recovery:
- Excellent for high-yield recovery of therapeutic proteins, enzymes, and other tagged molecules.
- Preliminary Protein Characterization:
- Supports preliminary steps in downstream applications such as SDS-PAGE, Western blotting, and mass spectrometry.
Handling and Storage
- Pre-charged Resin: Ready-to-use Ni-NTA resin is pre-charged with Ni²⁺ ions.
- Storage: Maintain at +4°C in its ethanol-containing slurry. Avoid freezing to prevent damage to the agarose matrix.
- Shelf Life: Stable for up to 6 months under recommended storage conditions.
Product Options
| Catalog Number | Unit Size |
|---|---|
| R90101 | 10 mL |
| R90115 | 25 mL |
| R90110 | 100 mL |
For Research Use Only. Not for use in diagnostic procedures.
- Pack Size: 25 mL 100 mL 10 mL
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