0
Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Human TIMP-2 ELISA Kit is a highly sensitive, quantitative sandwich ELISA assay designed for the accurate measurement of human Tissue Inhibitor of Metalloproteinases-2 (TIMP-2) in serum, plasma, and cell culture supernatants. TIMP-2 is a critical regulator of extracellular matrix remodeling and plays a key role in inhibiting matrix metalloproteinases (MMPs), including MMP-2 and MMP-9, making it an essential biomarker in cancer, cardiovascular disease, and inflammation research.
This kit offers exceptional analytical sensitivity of 2 pg/mL and a broad dynamic range of 2–1,200 pg/mL, enabling precise quantification of TIMP-2 across physiological and pathological levels. The assay utilizes a pre-coated 96-well microplate and optimized reagents to ensure reliable, reproducible results with minimal sample volume requirements.
Fully validated for sensitivity, precision, and lot-to-lot consistency, the Invitrogen TIMP-2 ELISA kit is ideal for biomarker discovery, molecular biology, and translational research applications.
Key Features and Advantages
Ultra-High Sensitivity and Wide Dynamic Range
- Analytical sensitivity: 2 pg/mL
- Assay range: 2–1,200 pg/mL
- Enables detection of low-abundance TIMP-2 protein
Highly Specific Sandwich ELISA Format
- Pre-coated capture antibody ensures high specificity
- Biotin-conjugated detection antibody with HRP signal amplification
- Accurate and reliable protein quantification
Minimal Sample Volume Requirement
-
Requires only:
- 1 µL serum or plasma
- 100 µL cell culture supernatant
- Conserves valuable research samples
Validated Performance and Precision
- Intra-assay CV: <10%
- Inter-assay CV: <12%
- Ensures reproducible experimental outcomes
Optimized Workflow and Efficiency
- Hands-on time: 1 hour 20 minutes
- Total assay time: 4 hours 45 minutes
- Compatible with standard absorbance microplate readers
Applications
The Human TIMP-2 ELISA Kit is ideal for research in:
- Cancer biology and tumor invasion
- Extracellular matrix remodeling studies
- Cardiovascular disease research
- Inflammation and immune response research
- Biomarker discovery and validation
- Cell culture protein expression analysis
- Molecular and cellular biology research
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Human TIMP-2 ELISA Kit |
| Brand | Invitrogen™ |
| Assay Format | Sandwich ELISA |
| Analytical Sensitivity | 2 pg/mL |
| Assay Range | 2–1,200 pg/mL |
| Sample Types | Serum, Plasma, Cell Culture Supernatant |
| Sample Volume | 1 µL (serum/plasma), 100 µL (supernatant) |
| Detection Method | HRP colorimetric detection |
| Detector Antibody | Biotin-conjugated |
| Plate Format | Pre-coated 96-well plate |
| Instrument Required | Absorbance microplate reader |
| Assay Time | 4 hr 45 min |
| Storage Conditions | 2–8°C (short term), −20°C (long term) |
| Shipping Conditions | Wet ice |
| Species Reactivity | Human |
| Research Use | Research Use Only (RUO) |
Kit Contents
- Pre-coated 96-well microplate
- TIMP-2 standards
- Assay diluent concentrate
- Biotinylated detection antibody
- Streptavidin-HRP conjugate
- Wash buffer
- Chromogen substrate
- Stop solution
- Adhesive plate covers
Ordering Information
| Catalog Number | Product Name | Number of Tests | Pack Size |
|---|---|---|---|
| EHTIMP2 | Human TIMP-2 ELISA Kit | 96 tests | 1 plate |
| EHTIMP2x5 | Human TIMP-2 ELISA Kit | 5 × 96 tests | 5 plates |
| EHTIMP2x10 | Human TIMP-2 ELISA Kit | 10 × 96 tests | 10 plates |
Target Information: TIMP-2
TIMP-2 is a key endogenous inhibitor of matrix metalloproteinases involved in:
- Regulation of extracellular matrix degradation
- Tumor invasion and metastasis control
- Tissue remodeling and repair
- Angiogenesis and inflammatory processes
TIMP-2 is widely studied as a biomarker in:
- Cancer progression
- Cardiovascular disease
- Fibrosis and tissue remodeling
- Inflammatory disorders
Unique Advantages
- Ultra-sensitive detection of human TIMP-2
- Validated and reproducible sandwich ELISA format
- Minimal sample volume requirement
- High specificity for natural and recombinant TIMP-2
- Ready-to-use reagents and optimized workflow
- Ideal for advanced biomarker research
The Invitrogen™ Human TIMP-2 ELISA Kit (EHTIMP2 series) provides a reliable, sensitive, and precise solution for quantifying TIMP-2 in human biological samples. With validated performance, low sample requirements, and robust assay design, it is an essential tool for research laboratories investigating extracellular matrix regulation, cancer progression, cardiovascular disease, and inflammatory processes.
- Pack Size: 10 x 96 Tests Plate 96 Tests Plate 5 x 96 Tests Plates