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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Human PGF ELISA Kit is a highly sensitive and specific sandwich ELISA assay designed for quantitative detection of human Placental Growth Factor (PlGF / PGF) in serum, plasma, urine, and cell culture supernatants. Placental Growth Factor is a key member of the VEGF family and plays a critical role in angiogenesis, vascular development, placental function, and tumor progression, making it an important biomarker in cancer, cardiovascular, and pregnancy-related research.
With an analytical sensitivity of 2 pg/mL and a dynamic range of 2–1,000 pg/mL, this kit enables precise quantification of PGF across physiological and pathological concentrations. The assay uses a pre-coated 96-well microplate and optimized reagents to ensure reliable, reproducible results for biomarker research and molecular biology applications.
Fully validated for sensitivity, specificity, and precision, the Invitrogen Human PGF ELISA kit provides a robust solution for angiogenesis and growth factor research.
Key Features and Advantages
Ultra-High Sensitivity and Wide Dynamic Range
- Analytical sensitivity: 2 pg/mL
- Assay range: 2–1,000 pg/mL
- Enables detection of low-abundance growth factor proteins
Highly Specific Sandwich ELISA Design
- Pre-coated capture antibody ensures specificity
- Biotinylated detection antibody with HRP amplification
- Accurate and reliable PGF protein quantification
Flexible Sample Compatibility
Compatible with multiple biological sample types:
- Serum (50 µL)
- Plasma (50 µL)
- Urine (50 µL)
- Cell culture supernatant (100 µL)
Validated and Reproducible Performance
- Intra-assay CV: <10%
- Inter-assay CV: <12%
- Ensures consistent and reliable results
Efficient Workflow
- Hands-on time: 1 hour 20 minutes
- Total assay time: 4 hours 45 minutes
- Compatible with standard absorbance microplate readers
Applications
The Human PGF ELISA Kit is ideal for research in:
- Angiogenesis and vascular biology
- Cancer and tumor progression research
- Cardiovascular disease research
- Pregnancy and placental biology research
- Biomarker discovery and validation
- Growth factor signaling studies
- Cell culture and protein expression analysis
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Human PGF ELISA Kit |
| Brand | Invitrogen™ |
| Assay Format | Sandwich ELISA |
| Analytical Sensitivity | 2 pg/mL |
| Assay Range | 2–1,000 pg/mL |
| Sample Types | Serum, Plasma, Urine, Cell Culture Supernatant |
| Detection Method | HRP colorimetric detection |
| Detector Antibody | Biotin-conjugated |
| Plate Format | Pre-coated 96-well plate |
| Instrument Required | Absorbance microplate reader |
| Assay Time | 4 hr 45 min |
| Storage Conditions | 2–8°C (short term), −20°C (long term) |
| Shipping Conditions | Wet ice |
| Species Reactivity | Human |
| Research Use | Research Use Only (RUO) |
Kit Contents
- Pre-coated 96-well microplate
- PGF standards
- Assay diluent concentrate
- Biotinylated detection antibody
- Streptavidin-HRP conjugate
- Wash buffer
- Chromogen substrate
- Stop solution
- Adhesive plate covers
Ordering Information
| Catalog Number | Product Name | Number of Tests | Pack Size |
|---|---|---|---|
| EHPGF | Human PGF ELISA Kit | 96 tests | 1 plate |
| EHPGFX5 | Human PGF ELISA Kit | 5 × 96 tests | 5 plates |
| EHPGFX10 | Human PGF ELISA Kit | 10 × 96 tests | 10 plates |
Target Information: Placental Growth Factor (PGF)
Placental Growth Factor (PlGF) is a key angiogenic protein involved in:
- Blood vessel formation (angiogenesis)
- Placental vascular development
- Tumor angiogenesis and metastasis
- Cardiovascular and inflammatory processes
PGF is widely used as a biomarker in:
- Cancer research
- Cardiovascular disease studies
- Pregnancy and placental biology research
- Angiogenesis and vascular biology research
Unique Advantages
- Ultra-sensitive detection of human PGF
- Validated sandwich ELISA for precise quantification
- Compatible with multiple biological sample types
- Reliable and reproducible results
- Optimized reagents for consistent performance
- Ideal for angiogenesis and growth factor research
The Invitrogen™ Human PGF ELISA Kit (EHPGF series) provides a reliable, sensitive, and efficient solution for quantifying Placental Growth Factor in human biological samples. With validated performance, high sensitivity, and optimized assay design, this kit is an essential tool for angiogenesis, cancer, cardiovascular, and placental biology research laboratories.
- Pack Size: 96 Tests Plate 5 x 96 Tests Plates 10 x 96 Tests Plates