Invitrogen™ Human FSH ELISA Kit

The Invitrogen™ Human FSH ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay for the detection and measurement of human follicle-stimulating hormone (FSH) in serum, plasma, and cell culture supernatants. Designed for research use, this kit reliably quantifies both natural and recombinant human FSH with high sensitivity and reproducibility.

Assay Principle

This kit uses a sandwich ELISA format with:

1. A pre-coated 96-well plate with an anti-human FSH capture antibody.
2. A biotinylated detection antibody specific to human FSH.
3. Streptavidin-HRP conjugate for signal amplification.
4. Chromogenic substrate (TMB) for color development, read at an appropriate wavelength using an absorbance microplate reader.

Key Features

• High Sensitivity – Detects as low as 8 pg/mL.
• Broad Assay Range – 8–2,000 pg/mL.
• Low Sample Volume – Requires only 50 μL per test.
• Reproducible Performance – Intra-assay CV <10%, Inter-assay CV <12%.
• Flexible Formats – Available in 96-test, 5 × 96-test, and 10 × 96-test pack sizes.

Applications

• Quantitative measurement of FSH in human serum or plasma.
• Assessment of pituitary gland function in research models.
• Studies of reproductive endocrinology.
• In vitro cell culture research involving gonadotropin regulation.

Technical Specifications

Kit Contents

• Pre-coated 96-well microplate
• Human FSH standard
• Assay diluent concentrate
• Biotinylated detection antibody
• Streptavidin-HRP conjugate
• Wash buffer
• Chromogen substrate
• Stop solution
• Adhesive plate covers

The Invitrogen™ Human FSH ELISA Kit delivers sensitive, specific, and reproducible quantitation of human FSH for research applications in endocrinology, reproductive biology, and pituitary function studies. With flexible formats and optimized reagents, it’s an ideal choice for laboratories conducting high-quality hormone analysis.