Invitrogen™ Human Calprotectin L1/S100-A8/A9 Complex ELISA Kit

The Invitrogen™ Human Calprotectin L1/S100-A8/A9 Complex ELISA Kit is a highly sensitive sandwich ELISA assay designed for the quantitative detection of calprotectin in human serum, plasma, and cell culture supernatants.

This assay specifically detects the S100A8/A9 heterocomplex (calprotectin), a key biomarker associated with neutrophil activation and inflammatory responses. With excellent sensitivity, reproducibility, and a wide dynamic range, the kit is ideal for immunology, inflammation, and clinical research applications.

Key Features and Benefits

High Sensitivity and Wide Dynamic Range

• Analytical sensitivity of 35 pg/mL
• Quantification range of 35–8,000 pg/mL
• Suitable for detecting low and high levels of calprotectin

Specific and Reliable Detection

• Recognizes both natural and recombinant human calprotectin
• Sandwich ELISA format ensures high specificity

Reproducible Performance

• Intra-assay CV: <10%
• Inter-assay CV: <12%

Optimized Workflow

• Hands-on time: approximately 1 hour 20 minutes
• Total assay time: ~4 hours 45 minutes
• Standard ELISA workflow compatible with common lab equipment

Flexible Sample Compatibility

• Validated for serum, plasma, and cell culture supernatants
• Low sample volume requirement: 50 µL per well

Applications

• Inflammation biomarker research
• Immunology and cytokine studies
• Neutrophil activation analysis
• Inflammatory bowel disease (IBD) research
• Infection and immune response studies
• Biomarker validation and translational research

Technical Specifications

Kit Contents

• Pre-coated 96-well microplate
• Calprotectin standard
• Assay diluent concentrate
• Biotinylated detection antibody
• Streptavidin-HRP (SAV-HRP)
• Wash buffer
• Chromogen substrate
• Stop solution
• Adhesive plate covers

Ordering Information

Storage and Handling

• Store at 2–8°C (short-term, up to 6 months)
• For long-term storage, maintain at -20°C
• Ship on wet ice
• Avoid repeated freeze-thaw cycles

Biological Background

Calprotectin (S100A8/A9 complex), also known as MRP-8/MRP-14, is a calcium-binding protein predominantly found in neutrophils, monocytes, and macrophages. It plays a critical role in immune responses, exhibiting antimicrobial, immunomodulatory, and chemotactic properties.

Elevated calprotectin levels are associated with inflammatory conditions, particularly those involving increased neutrophil activity, such as inflammatory bowel disease and systemic infections. Its presence in multiple biological fluids makes it a valuable biomarker for inflammation research.

Unique Advantages

• High sensitivity for low-abundance biomarker detection
• Specific detection of S100A8/A9 heterocomplex
• Reliable and reproducible ELISA performance
• Compatible with standard laboratory workflows
• Backed by Invitrogen™ quality and validation

The Invitrogen™ Human Calprotectin ELISA Kit provides a robust, sensitive, and reliable solution for quantifying calprotectin in human samples. Its precision and ease of use make it an essential tool for researchers investigating inflammation, immune responses, and disease biomarkers.