0
Specifications:
| Application | Fluorescence-Based Assay |
| Storage Temperature | Ambient |
| Product Type | Accessory |
| Product Brand | Thermo Fisher Scientific™, Invitrogen |
| Product Grade | Molecular Biology |
Invitrogen™ EVOS™ Light Cubes are high-performance, user-replaceable LED fluorescence illumination modules engineered for EVOS Imaging Systems and the Countess™ III FL Automated Cell Counter. The second-generation EVOS Light Cubes provide superior illumination uniformity, optimized spectral fidelity, and minimized bleed-through for unparalleled image quality and data accuracy.
Each cube integrates high-intensity LEDs, precision condensing optics, and hard-coated filters into a compact, digitally controlled light engine. The design ensures maximum excitation efficiency, superior signal-to-noise ratio, and consistent fluorescence intensity across the entire field of view, making them ideal for both bright and dim samples.
These light cubes are backward-compatible with first-generation EVOS and Countess systems, automatically recognized by the instrument, and can be swapped without tools, ensuring easy workflow integration.
Key Features & Benefits
- Exceptional Illumination Uniformity – Large-aperture design ensures even light distribution for improved tile-stitched imaging.
- Optimized Spectral Fidelity – Maximizes target fluorophore signal while reducing cross-talk between channels.
- High-Efficiency Hard-Coated Filters – Provide sharper cutoffs and >25% higher light transmission compared to soft-coated filters.
- Stable, Long-Life LEDs – Rated for >50,000 hours of continuous illumination, ensuring consistent performance and reproducibility.
- User-Friendly Operation – Light cubes are hot-swappable, automatically recognized, and digitally controlled.
- Reduced Photobleaching – Adjustable illumination intensity helps protect samples during extended imaging sessions.
- Lower Total Cost of Ownership – Eliminates frequent bulb replacements typical with mercury or metal halide lamps.
Model Specifications
| Catalog Number | Model | Excitation (nm) | Emission (nm) | Ideal For |
|---|---|---|---|---|
| AMEP4950 | DAPI 2.0 | 357/44 | 447/60 | BFP, DAPI, Hoechst, LysoTracker Blue, NucBlue Live |
| AMEP4951 | GFP 2.0 | 482/25 | 524/24 | Alexa Fluor 488, CellTracker Green, GFP, MitoTracker Green |
| AMEP4952 | RFP 2.0 | 542/27 | 593/40 | Alexa Fluor 555, Cy3, MitoTracker Orange CMTMRos, pHrodo, RFP, Rhod-2 |
| AMEP4954 | YFP 2.0 | 500/24 | 542/27 | EYFP, Venus, acridine orange |
| AMEP4955 | Texas Red 2.0 | 585/29 | 628/32 | Alexa Fluor 594, mCherry, MitoTracker Red CMXRos, mKate, Texas Red |
| AMEP4956 | Cy5 2.0 | 635/18 | 692/40 | Alexa Fluor 647, Cy5, DRAQ5, NucRed Live 647, SYTO 60 |
| AMEP4967 | Cy7 2.0 | 716/40 | 794/32 | Cy7, Alexa Fluor 750 |
Applications
- Multi-channel fluorescence microscopy
- Live-cell imaging and long-term culture observation
- Immunofluorescence staining
- Molecular biology and cell biology research
- Quantitative fluorescence imaging with minimal photobleaching
- Tile-stitch imaging for large specimen coverage
Product Specifications
| Parameter | Detail |
|---|---|
| For Use With | Countess™ III FL, EVOS™ M5000, EVOS™ M7000 |
| Includes | Integrated condensing optics and hard-coated filters |
| Light Source | High-intensity LED |
| LED Lifetime | >50,000 hours |
| Quantity | 1 cube |
| Product Line | EVOS |
| Type | LED Light Cube |
| Compatibility | Backward-compatible with first-generation EVOS and Countess systems |
| Unit Size | Each |
The Invitrogen™ EVOS™ Light Cubes (Second Generation) deliver precision-engineered fluorescence excitation for advanced microscopy applications. With unmatched spectral fidelity, long-life illumination, and high transmission efficiency, they enhance both image clarity and data reproducibility while offering a low-maintenance, cost-effective alternative to traditional light sources.
- Light Cube Type: GFP 2.0 DAPI 2.0 RFP 2.0 YFP 2.0 Texas Red 2.0 Cy5 2.0 Cy7 2.0