Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
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Specifications:
| Application | Cell culture | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Cell Culture | ||
Invitrogen™ EDTA (Ethylenediaminetetraacetic acid) is a 0.5 M aqueous solution at pH 8.0, prepared and certified RNase-free for sensitive molecular biology workflows. EDTA is a widely used chelating agent that sequesters divalent metal ions like Mg²⁺ and Ca²⁺, thus inhibiting enzymatic activities, particularly nucleases. This Ambion™ molecular biology-grade reagent is manufactured in nuclease-free facilities and rigorously tested to ensure zero detectable RNase, DNase, or endonuclease activity, making it ideal for RNA and DNA preservation and handling.
Key Features
- RNase-Free Certified – Ideal for RNA handling, protection, and processing
- 0.5 M, pH 8.0 – Optimal for most nucleic acid protocols
- Molecular Biology Grade – Free from endonucleases, exonucleases, and RNases
- Ready-to-Use – Supplied as a sterile-filtered solution in screw-cap bottles
- Manufactured in RNase-Free Facilities – Ensures reagent integrity and reproducibility
Specifications
| Property | Value |
|---|---|
| Chemical Name | EDTA (Ethylenediaminetetraacetic acid) |
| Concentration | 0.5 M |
| pH | 8.0 |
| Form | Liquid |
| Packaging Type | Bottle |
| Purity | RNase-free, Molecular Biology Grade |
| Product Line | Ambion™ |
| Unit Size | Each |
| Storage Conditions | Ambient (room temperature) |
| Shipping Conditions | Room temperature |
| For Use In | RNA/DNA extraction, enzyme inhibition |
| Regulatory Status | For Research Use Only (RUO) |
Applications
- RNA preservation and extraction workflows
- DNA sample stabilization
- Inhibition of divalent ion-dependent nucleases (e.g., DNase, RNase)
- Enzyme reaction buffering
- Molecular cloning and qPCR sample preparation
Ordering Information
| Catalog Number | Volume | Packaging Type |
|---|---|---|
| AM9260G | 100 mL | RNase-free bottle |
| AM9261 | 500 mL | RNase-free bottle |
| AM9262 | 1 L | RNase-free bottle |
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-Free is a dependable, high-purity reagent essential for protecting RNA and DNA integrity in molecular biology workflows. Manufactured and quality-tested to the highest standards, it supports sensitive experiments requiring nuclease-free conditions.
- Pack Size: 100 mL 500 mL 1 Liter
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